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OJVRTM
Online Journal of Veterinary
Research©
Volume 22 (5):362-368,
2018.
Effect of
calcium disodium EDTA and thiamine on subchronic lead
toxicity and oxidative DNA damage in dogs.
Maher Saber Owain,
Afaf Abdulrahman Yousif.
1Department of Internal and Preventive Veterinary Medicine, College of
Veterinary Medicine, University of Baghdad, Iraq.
ABSTRACT
Owain MS, Yousif AA., Effect of calcium disodium
EDTA and thiamine on subchronic lead toxicity and
oxidative DNA damage in dogs, Onl J Vet Res., 22 (5):362-368,
2018. Lead is a heavy dense metal inducing
toxicity especially in
young animals and children. We describe effects of calcium disodium and
thiamine on sub-chronic lead acetate drench in dogs assessed by serum malondialdehyde, gluthathione and
comet assay for DNA damage. Groups of 5 pound dogs each were drenched 15mg/kg lead
acetate daily for 60 days with or without calcium disodium EDTA (ca-EDTA)
110mg/Kg subcutaneously (SC) twice daily for 3 days, thiamine 25mg/Kg SC twice
daily for 7 days and 110mg/kg ca-EDTA 110mg/Kg SC twice daily for 3 days with 25mg/kg
thiamine SC twice daily for 7 days. Jugular blood was taken 15 days after the
end of the 60 day treatments for serum malondialdehyde,
glutathione and comet assay. We found lower glutathione in all dogs given lead
but the decrease was lower (P < 0.05) in dogs treated with calcium disodium
or thiamine. We observed a similar trend with malondialdehyde
which increased markedly in lead controls and less (P < 0.05) in dogs given
EDTA or thiamine. Our results show a showed a high repair rate of DNA after
treatment with thiamine and Ca-EDTA compared with lead controls. Glutathione
and malondialdehyde and comet assay are biomarkers for
lead exposure. We find that thiamine and Ca-EDTA protected dogs against
lead exposure.
Key words: Lead, Ca-EDTA, thiamine,
DNA, dog.
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