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Online Journal of Veterinary Research©
Volume 22 (5):409-416, 2018.
Humoral and cellular immune responses in lambs vaccinated with killed E. Coli O157:H7 vaccines.
1Ali Hussein Fadhil, 2Afaf Abdulrahman Yousif.
1Department(s). of Internal and Preventive Veterinary Medicine, College(s) of Veterinary Medicine, University of Karbala, 2University of Baghdad, Iraq. Corresponding Author : Afaf Abdulrahman Yousif. E. mail: firstname.lastname@example.org
Fadhil AH, Yousif AA., Humoral and cellular immune responses in lambs vaccinated with killed E. Coli O157:H7 vaccines,. Onl J Vet Res., 22 (5):409-416, 2018. We report humoral and cellular immune responses of lambs immunized by heat-killed E. coli O157:H7 vaccines with 1X108 or 1X1010CFU/ml. Three groups of 5 Arabi breed lambs each were injected subcutaneously (SC) with 2 ml of killed E coli O157:H7 vaccine with 1X108 CFU/ml mixed with equal volume of adjuvant at 2 week intervals, once with 1X1010 CFU/ml SC with adjuvant, and 2 ml sterile PBS mixed with equal volume of adjuvant twice (PBS controls). Another group of 5 were untreated controls. After vaccinations, lambs were monitored daily for clinical signs and a skin test was done at 21 days. Serum was taken 0, 7, 14, 21, 28, 42, 56, and 70 days for IgG determined by ELISA test. Lambs vaccinated with 1×1010 CUF /ml showed significant (p ≤ 0.05) increases in skin thickness compare with other groups by 48 hours. Mean serum IgG levels in vaccinated lambs increased significantly at (p ≤ 0.05) at day 7 and 14 after the first injection of vaccine and at days 28 and 42 post after a second booster injection. Serum IgG in controls remained unchanged until day 42. The findings suggest that killed 1X1010 CFU/ml E. coli O157 vaccine could protect sheep against effects of pathogenic E coli.
Key words: ELISA test, immunization, lambs, E. coli O157:H7.