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OJVRTM
Online Journal of Veterinary Research©
Volume 22 (5):409-416, 2018.
Humoral and cellular immune responses in lambs
vaccinated with killed E. Coli O157:H7 vaccines.
1Ali
Hussein Fadhil, 2Afaf Abdulrahman Yousif.
1Department(s). of Internal
and Preventive Veterinary Medicine, College(s) of Veterinary Medicine,
University of Karbala, 2University of
Baghdad, Iraq. Corresponding Author :
Afaf Abdulrahman Yousif.
E. mail: afaf_a.rahman@yahoo.com
ABSTRACT
Fadhil
AH, Yousif AA., Humoral and cellular immune responses
in lambs vaccinated with killed E.
Coli O157:H7 vaccines,. Onl
J Vet Res., 22 (5):409-416, 2018. We report humoral and cellular immune
responses of lambs immunized by heat-killed E. coli O157:H7 vaccines
with 1X108 or 1X1010CFU/ml. Three groups of 5 Arabi breed lambs each were injected subcutaneously (SC)
with 2 ml of killed E coli O157:H7 vaccine with 1X108 CFU/ml mixed with equal volume of adjuvant at 2 week intervals, once with 1X1010
CFU/ml SC with adjuvant, and 2 ml sterile PBS mixed with equal volume of
adjuvant twice (PBS controls). Another
group of 5 were untreated controls.
After vaccinations, lambs were monitored daily for clinical signs and a
skin test was done at 21 days. Serum was taken 0, 7, 14, 21, 28, 42, 56, and 70
days for IgG determined by ELISA test. Lambs vaccinated with 1×1010
CUF /ml showed significant (p ≤ 0.05)
increases in skin thickness compare with other groups by 48 hours. Mean serum IgG levels in vaccinated lambs increased
significantly at (p ≤ 0.05) at day 7 and 14 after the first injection of
vaccine and at days 28 and 42 post after a second booster injection. Serum IgG
in controls remained unchanged until day 42. The findings suggest that killed
1X1010 CFU/ml E. coli O157 vaccine could protect sheep against effects of pathogenic E coli.
Key words: ELISA
test, immunization, lambs, E. coli O157:H7.
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