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Online Journal of Veterinary Research

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X

Volume 26 (1):34-46, 2022.

Single nucleotide polymorphism in 5'-untranslated lactoferrin gene associated with uterine infections in cows


A Hajibemani1, H Sharifiyazdi1, A Mirzaei1, A Rowshan Ghasrodashti2


1Department of Clinical Science, School of Veterinary Medicine, Shiraz University, Shiraz 71345, Iran; 2Islamic Azad University, Kazerun Branch, Kazerun, Iran




Hajibemani A, Sharifiyazdi H, Mirzaei A, Ghasrodashti AR., Single nucleotide polymorphism in 5'-untranslated lactoferrin gene associated with uterine infections in cows, Onl J Vet Res., 26 (1):38-48, 2022. Lactoferrine (Lf) has antimicrobial effects in normal uterine discharges in cows and allele diversity in its gene may be associated with susceptibility or resistance to infection in its expression. We report single nucleotide polymorphism genesin 5' untranslated region (5'-UTR, position +32) of lactoferrin gene determined by allele-specific PCR in cows with or without uterine infection. Blood was taken from 89 multiparous Holstein dairy cows with a history of uterine infection (n = 51) and normal controls (n= 38). We found G > C polymorphisms in 5'-UTR area in all cows. By PCR, alleles correlated with sequencing (P < 0.05). However we found no correlation between disease and SNP in 5'-UTR. Cows with +32:CC and +32:GC genotypes with SNP in UTR had less coitus per conception and open days compared with those with +32:GG genotypes (p <0.05). However, we found no effect on calving to first service between genotypes. Our findings suggest that G to C transversion within 5'-UTR of bovine lactoferrin gene may b boost reproductive performance in dairy cows. The polymorphism in 5'-UTR of bovine lactoferrin gene might regulate expression, inhibit uterine infection and enhance embryo survival.


Keywords: Lactoferrin; Polymorphism; Uterine infection; Dairy cow.