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Online Journal of Veterinary Research©

 (Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X


Volume 26 (1):79-85, 2022.

Prevalence of paratuberculosis by serum interferon, interleukin ELISA and feces in buffalo (Iraq).


Nabeel Ahmed Al Anbagi1 , Sufyan Saleh Salman2


1Department of Veterinary Clinical Sciences, Faculty of Veterinary Medicine, University of Kufa, 2Department of Internal and Preventive Medicine, Faculty of Veterinary Medicine, University of Baghdad, Iraq




Al Anbagi NA, Salman SS., Prevalence of paratuberculosis by serum interferon, interleukin ELISA and feces in buffalo (Iraq), Onl J Vet Res., 26 (1):79-85, 2022.  We report prevalence of Johne’s disease by Mycobacterium avium subsp. Paratuberculosis (MAP) in 100 buffalo from Al Najaf, Iraq. One hundred blood and fecal samples were screened by Interferon (IFN)-γ, Interleukin-6 (IL6) ELISA and Ziehl-Nielsen tests. By Interferon gamma, we found 18% overall prevalence for MAP and by interleukin-6, 29%.  In farm buffalo we found 22% and abbatoir 36% prevalence with no significant differences. By 6 locations prevalence varied 0 to 33% without significant differences. By Ziehl-Nielsen fecal smears M. paratuberculosis appeared as nested red-pink short thick coccobacilli with 27% overall prevalence, 32% from farms and 22% from slaughter without significant differences. We found no effect of gender on prevalence of MAP due to small male cohort (10) compared with females (90). Similarly, by interferon gamma and interleukin -6 tests age had no significant effect on MAP. However, by fecal stain we did find a highly significant (P < 0.0001) effect of age with those 4-5 years with 59% incidence compared with 1 to 4 year old buffalo with 6-22%. Our results suggest that ELISA and fecal stains detected similar prevalence. We find relatively high prevalence for MAP in Iraq.


Key words: Mycobacteria, buffalo, prevalence, ELISA, INF-y, Interleukin, Iraq.