Volume
23 (6):509-515, 2019.
Prevalence of Toxoplasma
goondi in women pediatric outpatients with
history of abortion by TORCH, ELISA and nPCR tests.
Zuhair A. Ahmed, Abdullah H.
Abdullah
Faculty of Pharmacy , University of Anbar,
Faculty of Education for Women, University of Tikrit
ABSTRACT
Ahmed ZA, Abdullah AH., Prevalence of Toxoplasma goondi
in women pediatric outpatients with history of abortion by TORCH, ELISA and nPCR tests, Onl J Vet Res., 23 (6):509-515, 2019. We surveyed 150 Pediatric and Maternity hospital
outpatient women in Ramadi, Iraq from November 2017 to April 2018 for Toxoplasma gondii by direct smear, TORCH, ELISA and PCR.
We detected 88 infected samples assigned by medical history to groups of infected with abortion, pregnant,
not-pregnant or normal birth and not-infected
with normal birth, not-pregnant-aborted groups. We found agreement between nPCR (~81%), ELISA
IgG (80%) and smear (68%) for T. gondii but TORCH Cassette test was least accurate
(31%). IgM was negative for all samples. Serum IgG, complement and IL-8
interleukin values were consistently higher in infected patients compared with
other groups even if they had aborted or were pregnant or not. Serum IgG was ~84%
and complement 3, 12 to ~34% higher (P < 0.05) in women with toxoplasma gondii.
We found higher incidence for T goondi in women residing in rural areas (81.25%) and slightly less in urban
locations (77.5%). Our results also suggested higher incidence in 26-35 year
olds (~94.6%) but lower in older women (36-45y) (20%)
Key Words: IL. (Interlukin), nPCR(Nested),
ELISA (Enzyme Link Immunosorbent Assay). Raw data provided after reference list.
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