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OJVRTM

Online Journal of Veterinary Research

Volume 23 (6):509-515, 2019.


Prevalence of Toxoplasma goondi in women pediatric outpatients with history of abortion by TORCH, ELISA and nPCR tests.

 

Zuhair A. Ahmed, Abdullah H. Abdullah

 

Faculty of Pharmacy , University of Anbar, Faculty of Education for Women, University of Tikrit

 

ABSTRACT

 

Ahmed ZA, Abdullah AH., Prevalence of Toxoplasma goondi in women pediatric outpatients with history of abortion by TORCH, ELISA and nPCR tests, Onl J Vet Res., 23 (6):509-515, 2019. We surveyed 150 Pediatric and Maternity hospital outpatient women in Ramadi, Iraq from November 2017 to April 2018 for Toxoplasma gondii by direct smear, TORCH, ELISA and PCR. We detected 88 infected samples assigned by medical history to groups of infected with abortion, pregnant, not-pregnant or normal birth and not-infected with normal birth, not-pregnant-aborted groups. We found agreement between nPCR (~81%), ELISA IgG (80%) and smear (68%) for T. gondii but TORCH Cassette test was least accurate (31%). IgM was negative for all samples. Serum IgG, complement and IL-8 interleukin values were consistently higher in infected patients compared with other groups even if they had aborted or were pregnant or not. Serum IgG was ~84% and complement 3, 12 to ~34% higher (P < 0.05) in women with toxoplasma gondii. We found higher incidence for T goondi in women residing in rural areas (81.25%) and slightly less in urban locations (77.5%). Our results also suggested higher incidence in 26-35 year olds (~94.6%) but lower in older women (36-45y) (20%)

 

Key Words: IL. (Interlukin), nPCR(Nested), ELISA (Enzyme Link Immunosorbent Assay). Raw data provided after reference list.


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