Volume 23 (1):66-74, 2019.
Prevalence of Cryptosporidium parvum, hematology and immune markers in
hospitalized children and pathogenicity in Swiss mice.
Hanan Yousif Rasheed Alharby, Thaer Abdulqader Salih Alalusi
Obstetrics and Gynecology Hospital, Ramadi, Anbar City, Iraq.
Rasheed Alharby HY, Salih Alalusi TA, Prevalence of Cryptosporidium parvum, hematology and immune markers in hospitalized children and pathogenicity in Swiss mice, Onl J Vet Res., 23 (1):66-74, 2019. Cryptosporidium parvum, was isolated from feces of male and female children <10 years age. To assess pathology, the C parvum oocytes were gavaged in groups of Swiss mice. Of 300 feces of children tested, we detected C parvum in ~7%-13% feces by various methods with highest incidence by ELISA (40 children). We found higher prevalence in males (15.6%) than females (10.7%), aged 0-1y, ~29% and 6 to 9y 3.9%, from rural areas ~15% and cities ~9%. Compared with non-infected controls, red blood cells were ~ 2% lower, white cells 21% and platelets 17% higher, cholesterol 15%, triglyceride 21% and low and very low density lipoprotein 23-29% higher and protein 6%, albumin 12% and globulin 4% lower. In infected children values for complement 4 were +36% higher, C3 27%, IgA 43% and interleukin L2, 33% higher. Significance for differences between infected and non-infected data ranged P < 0.05 to <0.0001. In mice gavaged C parvum, we confirmed pathogenicity by observing infiltration by neutrophils, vacoulation, hemorrhage, and autolysis of structures with various stage of C parvum inside intestinal tissues. Our results suggest that in young children, C parvum mainly affected blood antibody immune markers and lipids.
Keywords: Cryptosporidium, children, hematology, immune markers, pathogenicity, mice.