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OJVRTM
Online Journal of
Veterinary Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume
28(4): 240-245, 2024.
Diagnosis of
tuberculosis by g-interferon with interleukin-2 receptors
assay.
Ritelli M, Amadori M, Dondo A, Begni B, Zoppi S, Archetti IL.
ABSTRACT
Ritelli M, Amadori M, Dondo A, Begni B, Zoppi S,
Archetti IL. Diagnosis of tuberculosis by g-interferon and interleukin-2 receptor assay, Onl J Vet Res., 28(4): 240-245, 2024. The g-interferon assay for bovine tuberculosis can be validated
further by expression of interleukin 2 receptor tests. Tuberculosis-positive
herds validated by PPD tuberculin, necropsy and bacteriology were subjected to
both tests. Seventy-five out of 765 samples were g-interferon positive (9.8%)
and 9 were dubious (1.18%). We found 73/565 g-interferon positive in one herd,
0/57 and 2/143 in other herds. Then we tested 78, 11 and 6 cattle in same herds
by the IL-2R assay and found 72 g-interferon positive, 14 negative and 9
dubious. Sixty four cattle yielded the same result in both tests. Eighty of 95
cattle were slaughtered for necropsy and we found 38 TB lesion-positive of
which 36 were g-interferon positive and 2 dubious. As for the IL-2 R assay, 33
were positive, 1 negative and 4 dubious. No g-interferon negative animal scored
positive in the IL-2R assay but 10 g-interferon positive and dubious samples
scored negative in the IL-2R assay; one being TB lesion-positive with highest
background expression of IL-2 receptor. Results of interleukin-2 receptor were
highly correlated (P < 0.001) with g-interferon
assay. However we found no increase in specificity.
KEYWORDS:
Cattle, Tuberculosis, Diagnosis, g-interferon, interleukin-2
receptor.