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Online Journal of Veterinary Research©

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X


Volume 28(4): 240-245, 2024.

Diagnosis of tuberculosis by g-interferon with interleukin-2 receptors assay.

 Ritelli M, Amadori M, Dondo A, Begni B, Zoppi S, Archetti IL.


Department of Immunoprophylaxis and Animal Welfare, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia-Romagna, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Italia.  




Ritelli M, Amadori M, Dondo A, Begni B, Zoppi S, Archetti IL. Diagnosis of tuberculosis by g-interferon  and interleukin-2 receptor assay, Onl J Vet Res., 28(4): 240-245, 2024. The g-interferon assay for bovine tuberculosis can be validated further by expression of interleukin 2 receptor tests. Tuberculosis-positive herds validated by PPD tuberculin, necropsy and bacteriology were subjected to both tests. Seventy-five out of 765 samples were g-interferon positive (9.8%) and 9 were dubious (1.18%). We found 73/565 g-interferon positive in one herd, 0/57 and 2/143 in other herds. Then we tested 78, 11 and 6 cattle in same herds by the IL-2R assay and found 72 g-interferon positive, 14 negative and 9 dubious. Sixty four cattle yielded the same result in both tests. Eighty of 95 cattle were slaughtered for necropsy and we found 38 TB lesion-positive of which 36 were g-interferon positive and 2 dubious. As for the IL-2 R assay, 33 were positive, 1 negative and 4 dubious. No g-interferon negative animal scored positive in the IL-2R assay but 10 g-interferon positive and dubious samples scored negative in the IL-2R assay; one being TB lesion-positive with highest background expression of IL-2 receptor. Results of interleukin-2 receptor were highly correlated (P < 0.001) with g-interferon assay.  However we found no increase in specificity.


KEYWORDS: Cattle, Tuberculosis, Diagnosis, g-interferon, interleukin-2 receptor.