©2021-2033. All Rights Reserved. Online Journal of Veterinary Research . You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking:To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page.
Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Volume 27 (5): 267-272, 2023.
Bovine tuberculosis g-interferon and interleukin-2 receptor tests in cattle.
Ritelli M, Amadori M, Dondo A, Begni B, Zoppi S, Archetti IL.
Immunoprophylaxis and Animal Welfare, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia-Romagna, Brescia and Istituto Zooprofilattico Sperimentale del Piemonte, Turin, Italy.
Ritelli M, Amadori M, Dondo A, Begni B, Zoppi S, Archetti IL. Bovine tuberculosis g-interferon and interleukin-2 receptor tests in cattle. Onl J Vet Res., 27 (5): 267-272, 2023. Inconclusive g-interferon receptor tuberculosis tests can be resolved by inducing expression of interleukin-2 receptor as immune marker for M. bovis on same blood sample. Seventy-five of 765 samples were g-interferon positive (9.8%), 9 dubious (1.18%) and 14 negative. Sixty four cattle had same result by both tests (67.4%). Of 95 cattle slaughtered, 38 were TB lesion-positive with 36 g-interferon positive and 2 dubious. By IL-2 R assay, 33 scored positive, 1 negative and 4 dubious. No g-interferon negatives scored positive by IL-2R assay but 10 g-interferon positive and dubious samples scored negative. Forty-two g-interferon positive and 34 dubious M. bovis-positive or suspect farms tested by IL-2R assay yielded 16 SICT-positive. Thirty-five of 42 g-interferon positive were positive by IL-2R assay, 5 negative and 2 dubious. Twenty g-interferon dubious animals were scored positive but 10 negative by IL-2R assay and 4 dubious. By SICT 13 of 16 were positive in IL-2R assay (81%). We found no discrepancy with IL-2R assay for g-interferon negative cattle. Blood from M. bovis-positives appeared negative by g-interferon or IL-2R assays. We find both tests to have low specificity but high agreement.
KEYWORDS: Cattle, tuberculosis, tests, g-interferon, interleukin-2, receptors.