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Online Journal of Veterinary Research©

(Including Medical and Laboratory Research)

Established 1994
ISSN 1328-925X


Volume 27 (5): 267-272, 2023.

 Bovine tuberculosis g-interferon and interleukin-2 receptor tests in cattle.


 Ritelli M, Amadori M, Dondo A, Begni B, Zoppi S, Archetti IL.


Immunoprophylaxis and Animal Welfare, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia-Romagna, Brescia and Istituto Zooprofilattico Sperimentale del Piemonte,  Turin, Italy.  



Ritelli M, Amadori M, Dondo A, Begni B, Zoppi S, Archetti IL. Bovine tuberculosis g-interferon and interleukin-2 receptor tests in cattle. Onl J Vet Res., 27 (5): 267-272, 2023.   Inconclusive g-interferon receptor tuberculosis tests can be resolved by inducing expression of interleukin-2 receptor as immune marker for M. bovis on same blood sample. Seventy-five of 765 samples were g-interferon positive (9.8%), 9 dubious (1.18%) and 14 negative. Sixty four cattle had same result by both tests (67.4%). Of 95 cattle slaughtered, 38 were TB lesion-positive with 36 g-interferon positive and 2 dubious. By IL-2 R assay, 33 scored positive, 1 negative and 4 dubious. No g-interferon negatives scored positive by IL-2R assay but 10 g-interferon positive and dubious samples scored negative. Forty-two g-interferon positive and 34 dubious M. bovis-positive or suspect farms tested by IL-2R assay yielded 16 SICT-positive. Thirty-five of 42 g-interferon positive were positive by IL-2R assay, 5 negative and 2 dubious. Twenty g-interferon dubious animals were scored positive but 10 negative by IL-2R assay and 4 dubious. By SICT 13 of 16 were positive in IL-2R assay (81%). We found no discrepancy with IL-2R assay for g-interferon negative cattle. Blood from M. bovis-positives appeared negative by g-interferon or IL-2R assays. We find both tests to have low specificity but high agreement.

 KEYWORDS: Cattle, tuberculosis, tests, g-interferon, interleukin-2, receptors.