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OJVRTM
Online Journal of Veterinary Research©
Volume 18(9):
701-709, 2014.
Molecular characterization of class
1 integrons and antibiotic resistance to E. coli from cattle, sheep and goats.
Yahya Tahamtan1*, Masood Asadi Fozi2, Mohammad Hossein Hosseini1,
Tahereh Mostafaee2
1Department of Bacteriology, Razi Vaccine and Serum Research Institute-Shiraz 2Animal
science, Faculty of Agriculture Shahid Bahonar University of Kerman, Iran
ABSTRACT
Tahamtan
Y, Asadi Fozi M, Hosseini MH,
Mostafaee T., Molecular characterization of class 1 integrons and antibiotic resistance in cattle, sheep and
goats E. coli, Onl J Vet Res., 18(9): 701-709, 2014. E.coli infection
is a major health problem especially among animal and children in developing
countries. Moreover, E. coli is
becoming resistant to commonly used antimicrobials in most parts of the world
Molecular characterization of
class 1 integrons and prevalence of antibiotic
resistance to E. coli from cattle, sheep and goats is reported. The study was
conducted during 2013. Swab stool specimens were collected from 250 cattle,
sheep and goats from different parts of Fars province in Iran. Isolation and
characterization were performed according to standard methodology including biochemical
test for identification of E. coli and PCR for detection of the integron gene in isolates. 173 E. coli strains were
isolated, all of which were resistant to at least one antibiotic. 100% were
resistant to amoxicillin,
penicillin, tetracycline, ampicillin and co-trimoxazole, 92% to erythromycin and neomycin, 88% to gentamicin and 84% to amikacin.
More than 50% were susceptible to sulfamethoxazole, enorfloxacin and cefalexin. Prevalence of integrons in the E. coli isolates was 34% and lack
of antibiotic susceptibility to antibiotics was associated with its presence in
bacteria. E.
coli may select a specific gene cassette through antibiotic selective
pressure resulting in class 1 integron differences.
Horizontal transfer through conjugative plasmids could be responsible for
dissemination for class 1 integrons.
Keywords: class 1 integrons, antibiotic
resistance, cattle, sheep, goats, E.
coli.
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