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Online Journal of Veterinary Research©
Volume 27 (6): 353-356, 2023
Replication of H9N2 avian influenza virus in lung and kidney of broilers
Department of Avian Medicine, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran
Tavakkoli H., Replication of H9N2 avian influenza virus in lung and kidney of broilers, Online J Vet Res., 27 (6): 353-356, 2023. Author reports replication of H9N2 avian influenza virus in in lung and kidney of 30 day broilers by 2-step TaqMan real time PCR assay after intranasal inoculation. Groups of 5 chicks each viral titers in lung on day 3 was 12338 (20%), day 7 376570 (40%), day 11 94800 (20% and day 15, 0. In kidneys these values were 106 (20%), 73900 (80%), 2484 (20%) and 6436 (20%) respectively. PCR showed that H9N2 titers in lungs and kidneys 3 days after challenges were lower (P 0.05) than at days 7 and 11 with highest titers in lungs.
Keywords: Avian influenza, H9N2, TaqMan real-time PCR, Virus quantitation.