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OJVRTM

 

Online Journal of Veterinary Research©

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X

 

Volume 29 (4): 198-210, 2025.

Prevalence of avian influenza H9, bronchitis and vaccine viruses in broilers.

 

Seifi S, Asasi K, Mohammadi A.

 

Group of Veterinary Medicine, Islamic Azad University, Poultry Research Center and Pathobiology,  School of Veterinary Medicine, Shiraz University, Iran

 

ABSTRACT

 

Seifi S, Asasi K, Mohammadi A, Prevalence of avian influenza H9, bronchitis and vaccine viruses in broilers, Onl J Vet Res, 29 (4): 198-210, 2025. We report prevalence of natural avian influenza virus (AIV) H9N2 and infectious bronchitis (IBV) viruses in broilers from Fars, Iran. Tracheas were swabbed from 30 broiler flocks with respiratory distress and high mortality. Multiplex RT-PCR was done to detect M protein and H9 gene in AIV as well as Massachusetts, 793B (4/91) and D274 serotypes of IBV. Of 30 flocks, we found AIV in 16 (53/3%) and IBV in 12 (40%). Eleven of 12 flocks were positive for IBV 4/91 but only 1 for Massachusetts serotype. Mixed infections of AIV H9 and IBV 4/91 were observed in 4 flocks with severe lesions and 20-30% mortality. Findings suggest high incidence natural co-infection of AIV and IBV in broilers and those with respiratory distress resulted in highest mortality (P < 0.05).  

 

Key words: Avian Influenza, Infectious Bronchitis, Vaccine, viruses, Co- infection.

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