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Online Journal of Veterinary Research

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X

Volume 27 (12):664-669, 2023.

Processing M2e-HA2 influenza A virus epitope from Escherichia coli for vaccine.


Masoud Moghadaszadeh, Golchin M, Tavakkoli H, Mehdi Gol Reza Ghanbarpour.


School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.




Moghadaszadeh M, Golchin M, Tavakkoli H, Ghanbarpour R., Processing M2e-HA2 influenza A virus epitope from Escherichia coli for vaccine, Onl J Vet Res., 27 (12): 664-669, 2023. High mutation rate of influenza viruses, obfuscates production of efficient vaccines, However, the viruses have conserved epitopes matrix-2 protein ectodomain M2e and HA2. We expressed and purified M2e-HA2 peptides influenza genes in Escherichia coli synthesized and cloned into pGS21vector. This construct was transferred into E. coli BL21 (DE3) and induced by IPTG. Expression of recombinant peptide was confirmed by western blot assay anti-GST monoclonal antibody. The expressed peptide-GST was purified from bacterial lysate by IMAC chromatography. Sequencing revealed that M2e-HA2 gene cloned in vector. SDS-PAGE electrophoresis revealed prominent and Western blot single bands for potential vaccine.


Key words: Cloning, Expression, Purification, M2e-HA2, Escherichia coli, Influenza vaccine.