2022-2032 All Rights Reserved. Online Journal of Veterinary Research . You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR.This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking:To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page.


OJVRTM
Online Journal of Veterinary Research

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X


Volume 27 (12):664-669, 2023.


Processing M2e-HA2 influenza A virus epitope from Escherichia coli for vaccine.

 

Masoud Moghadaszadeh, Golchin M, Tavakkoli H, Mehdi Gol Reza Ghanbarpour.

 

School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.

 

ABSTRACT

 

Moghadaszadeh M, Golchin M, Tavakkoli H, Ghanbarpour R., Processing M2e-HA2 influenza A virus epitope from Escherichia coli for vaccine, Onl J Vet Res., 27 (12): 664-669, 2023. High mutation rate of influenza viruses, obfuscates production of efficient vaccines, However, the viruses have conserved epitopes matrix-2 protein ectodomain M2e and HA2. We expressed and purified M2e-HA2 peptides influenza genes in Escherichia coli synthesized and cloned into pGS21vector. This construct was transferred into E. coli BL21 (DE3) and induced by IPTG. Expression of recombinant peptide was confirmed by western blot assay anti-GST monoclonal antibody. The expressed peptide-GST was purified from bacterial lysate by IMAC chromatography. Sequencing revealed that M2e-HA2 gene cloned in vector. SDS-PAGE electrophoresis revealed prominent and Western blot single bands for potential vaccine.

 

Key words: Cloning, Expression, Purification, M2e-HA2, Escherichia coli, Influenza vaccine.


MAIN

 

FULL-TEXT (SUBSCRIPTION OR PURCHASE TITLE)