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OJVRTM
Online Journal of
Veterinary Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 29 (1): 1-6, 2025.
Detection of Oesophagostonum columbianum in goats by indirect
Elisa
Bashir Ahmad Lone, MZ Chishti, Fayaz Ahmad, Hidayatullah Tak.
Department of Zoology, Faculty
Veterinary Science, University of Kashmir, Srinagar.
ABSTRACT
Lone BA,
Chishti MZ, Ahmad F, Tak H., Detection of Oesophagostonum columbianum in
goats by indirect Elisa, Onl J Vet Res., 29 (1): 1-6, 2025.
We report seroprevalence
of anti- Oesophagostomum
columbianum antibodies in 198 goats. ELISA was standardized to detect
somatic whole adult antigen of Oesophagostomum columbianum. Plate
ELISA was standardized in 5µg/well antigen with 1:100 and 1:1000 sera conjugate
dilution. Suspect field sera by ELISA
yielded 68 (34.34%) and 12 by fecal examination. Highest prevalence 52%
occurred in goats <1 yearcompared with >4 years
with 17% (P < 0.03). Gender was not related with infection Sensitivity of
ELISA was ~80% and specificity ~21%, whereas fecal tests yielded only 11 and
90%, respectively.
KEY-WORDS: ELISA, Oesophagostomum columbianum, Goat, Somatic antigen.
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