1994-2018 All Rights Reserved. Online Journal of Veterinary Research. You may
not store these pages in any form except for your own personal use. All other
usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other
way besides the before mentioned must be gained in writing from the publisher. This
article is exclusively copyrighted in its entirety to OJVR publications. This
article may be copied once but may not be, reproduced or re-transmitted without
the express permission of the editors.
OJVRTM
Online Journal of Veterinary Research©
Volume 22 (4):288-289, 2018.
Frequency of Entamoeba histolytica genotypes in diarrheic patients determined by nested RT-PCR’s.
Russul Wassit
Kadhum1, Nawar Jaber
Hussein Al–Asadi2, Baraa Abdulsalam Hraiga3
1Department of
Biology, College of Science, 2Microbiology, 3College of
Medicine, Wasit University, Kut,
Iraq.
ABSTRACT
Kadhum
RW, Hussein Al–Asadi NJ, Hraiga
BA., Frequency of Entamoeba histolytica genotypes in
diarrheic patients determined by nested RT-PCR’s, Onl
J Vet Res., 22 (4):288-289,
2018. A nested
PCR for detection of Entamoeba histolytica from 50 patients in Wasit,
Iraq with diarrhea from stools samples by 2 amplifications of a sub-unit RNA is
described. Thirty stools were found to be positive and subjected to another RT-PCR to
identify genotypes using specific primers polymorphic for serine rich E. histolytica protein (SREHP). In a 1st
run, a conventional PCR
amplified a 549-bp fragment of the SREHP gene. In the 2nd run, a
nested RT-PCR was done with SYBR Green I. Genotypes were identified by SYBR Green I melting
curve analysis. We
found 5 different genotypes classified as I to V with frequencies of II 46.67%,
I 13.33%, III and ӀV 16.67% and V only 6.7%. Our nested and RT-PCR’s
may be an alternative method for rapid screening for amebiasis.
Keywords:
Molecular detection; Genotyping; Entamoeba histolytica.
FULL-TEXT (SUBSCRIPTION OR
PURCHASE ARTICLE)