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Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Volume 27 (8): 484-490, 2023
Heterogenicity of Staphyloccocus aureus mastitis coagulase gene.
Zahra Esmailnezhad, Masoud Haghkhah, Narjes Ghaffari.
School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Esmailnezhad Z, Haghkhah M, Ghaffari N., Heterogenicity of Staphyloccocus aureus mastitis coagulase gene, Onl J Vet Res., 27 (9): 484-490, 2023. Coagulase phenotypes can identify virulence of S. aureus genotype strains. We report coagulase gene isolated from 40 mastitis S. aureus cows by routine biochemistry confirmed by PCR to yield 1.3kb products. Amplification of coagulase gene from all isolates yielded single bands of 700 (30%) and 900 bp (70%) exclusively bovine, suggesting that humans do not contaminate cow mammary glands. Digestion of coagulase gene PCR products with restriction endonuclease enzymes AluI and Hin61 yielded 3 different RFLP profiles that indicated a low heterogeneity in coagulase gene.
Key words: Mastitis, staphylococcus, coagulase, genes, heterogeneity.