©1996-2018. All Rights
Reserved. Online Journal of Veterinary Research . You may not store
these pages in any form except for your own personal use. All other usage or
distribution is illegal under international copyright treaties. Permission to
use any of these pages in any other way besides the
before mentioned must be gained in writing from the publisher. This
article is exclusively copyrighted in its entirety to OJVR.This
article may be copied once but may not be, reproduced or
re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements
(DEST) for the Higher Education Research Data Collection (Australia). Linking:
:To link to this page or any pages linking to this page you must link
directly to this page only here rather than put up your own page.
OJVRTM
Online Journal of Veterinary
Research ©
15 (3): 283-290, 2011. Redacted
2017.
Effect of intranasal live
gdhA
derivative P. multocida
B:2 vaccine on herd immunity in buffaloes.
Rafidah
O1,2, Zamri-Saad
M1, Nasip E2, Saharee AA1
1Faculty of Veterinary Medicine, Universiti
Putra Malaysia, and 2Department of Veterinary Services and Animal
Industry, Kota Kinabalu, Sabah, Malaysia
Abstract
Rafidah O, Zamri-Saad
M, Nasip E, Saharee AA.,
Effect of intranasal live gdhA derivative P. multocida B:2 vaccine on herd immunity in buffaloes. Onl J Vet Res., 15 (3): 283-290, 2011.
Effects of
a live attenuated gdhA derivative P.
multocida B:2 vaccine on immunity in buffalo
herds are described. Three groups of 30 to 50
buffaloes selected from a haemorrhagic septicaemia-endemic and non-endemic area were used. Thirty
percent
of animals in each group were given intranasal 5 ml inoculum 106 cfu/mL live attenuated gdhA
derivative P. multocida B:2. After exposure, vaccinated buffaloes were mingled
with non-vaccinated animals of the same group. A booster dose was given 10
months after the first exposure. Serum samples were randomly collected from 30%
of each group before vaccination and at 2-month intervals for 22 months to
determine herd immune status by ELISA. Endemic or non-endemic herd status was
compared with 15 non-vaccinated controls. Results revealed high herd antibody
levels in endemic and non-endemic areas before vaccination. After vaccination,
herd antibody levels in both areas gradually increased to peak values by the 6th
month and then started to gradually decline until month 10. Following booster
dose at 10th month, antibodies declined to levels similar to those
in unvaccinated animal at 12 to 14 months. Antibody levels then recovered at 22-months
(end of study). It was concluded that field vaccination using gdhA derivative P. multocida B:2
increased herd immunity for 8 to 10
months before a booster dose was required.
Key words: gdhA derivative Pasteurella multocida B:2;
immune response; field buffalo