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OJVRTM
Online Journal of Veterinary
Research©
Volume
23 (11):1119-1136, 2019.
Formulation and effect of vitamin K and
erythropoietin loaded hemosome on hemorrhagic anemia
in rabbits.
Mohanad A Albayati and Safaa Saad Alsamarraie.
Department of Physiology, Biochemistry
and Pharmacology, College of Veterinary Medicine, University of Baghdad.
ABSTRACT
Albayati MA, Alsamarraie
SS., Formulation and effect of vitamin
K and erythropoietin loaded hemosome on hemorrhagic
anemia in rabbits, Onl J Vet Res., 23 (11):1126-1140, 2019. We report effect of hemosome artificial
erythrocyte loaded with vitamin K and erythropoietin
on hematology in rabbits before, at 24h and 5 days after an induced hemorrhage.
Groups of 5 rabbits each were injected IV of hemosomes with or without 0.21mg/kg vitamin K or
925IU/kg Erythropoietin. Controls were given empty hemosome.
Blood was taken before, at 24h and 5 days later for hematology. Mean
encapsulation (%) for empty hemosome was 67%, with
vitamin K 75%, erythropoietin 75% or both 83% with efficiency of 56, 50, 68 and 63%, respectively. Compared
with blood taken before exsanguination, by 24h, hemoglobin had declined 38%,
red cells 50% and platelets 70% in all groups but by 5 days returned to normal
except in untreated controls with similar trends for packed cell and
corpuscular volumes. However, in rabbits injected erythropoietin we found large
increases in corpuscular hemoglobin and nascent erythroid hyperplasia. Clotting
times returned to normal in those given hemosomes
with vitamin K or erythropoietin, but did not recover in controls. We find that
liposomes loaded with vitamin K or erythropoietin may enhance blood production
in rabbits by 5 days after exsanguination.
Keywords: Hemosome, erythrosome, liposome-encapsulated
hemoglobin, hemorrhagic anemia, vitamin K, erythropoietin.