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Online Journal of Veterinary Research

 Volume 23 (11):1119-1136, 2019.

Formulation and effect of vitamin K and erythropoietin loaded hemosome on hemorrhagic anemia in rabbits.


Mohanad A Albayati and Safaa Saad Alsamarraie.


Department of Physiology, Biochemistry and Pharmacology, College of Veterinary Medicine, University of Baghdad.




Albayati MA, Alsamarraie SS., Formulation and effect of vitamin K and erythropoietin loaded hemosome on hemorrhagic anemia in rabbits, Onl J Vet Res., 23 (11):1126-1140, 2019. We report effect of hemosome artificial erythrocyte loaded with vitamin K and erythropoietin on hematology in rabbits before, at 24h and 5 days after an induced hemorrhage. Groups of 5 rabbits each were injected IV of hemosomes with or without 0.21mg/kg vitamin K or 925IU/kg Erythropoietin. Controls were given empty hemosome. Blood was taken before, at 24h and 5 days later for hematology. Mean encapsulation (%) for empty hemosome was 67%, with vitamin K 75%, erythropoietin 75% or both 83% with efficiency of 56, 50, 68 and 63%, respectively. Compared with blood taken before exsanguination, by 24h, hemoglobin had declined 38%, red cells 50% and platelets 70% in all groups but by 5 days returned to normal except in untreated controls with similar trends for packed cell and corpuscular volumes. However, in rabbits injected erythropoietin we found large increases in corpuscular hemoglobin and nascent erythroid hyperplasia. Clotting times returned to normal in those given hemosomes with vitamin K or erythropoietin, but did not recover in controls. We find that liposomes loaded with vitamin K or erythropoietin may enhance blood production in rabbits by 5 days after exsanguination.


Keywords: Hemosome, erythrosome, liposome-encapsulated hemoglobin, hemorrhagic anemia, vitamin K, erythropoietin.