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Online Journal of Veterinary Research


Volume 22 (2): 99-103, 2018.

Effect of two hour halothane anesthesia on blood cell counts and serum biochemical values in sheep and calves.


Rajaian H., DVM, PhD; Nazifi S., DVM, PhD; Nayyeri K., DVM; Saberi Afshar F., DVM and Ehteshami F., DVM.



School of Veterinary Medicine, Shiraz University, Shiraz, Iran




Rajaian H, Nazifi S, Nayyeri K, Saberi Afshar F, Ehteshami F., Effect of two hour halothane anesthesia on blood cell counts and serum biochemical values in sheep and calves, Onl J Vet Res., 22 (2): 99-103, 2018. Anesthesia was induced and maintained 2 hours by halothane in 5 sheep and 4 calves by closed circuit anesthetic machine. Average induction time was ~5 minutes in both groups. White blood cell count, packed cell volumes (PCV) blood urea nitrogen and serum glucose, cholesterol, total protein, protein fractions, creatine phosphokinase, alanine and aspartate transaminases, sodium and potassium were determined. Liver biopsies were taken two weeks after anesthesia10, 11 and were examined for any pathological changes using light microscopy. In sheep, blood PCV increased 1 and 7 days after induction and neutrophils by 2 hours whereas cholesterol declined 1-2 hrs. Pre-anesthetic serum cholesterol level was ~3 times greater in calves compared with sheep. There were no significant changes in any parameter in calves. Liver biopsies taken from sheep or calves 2 weeks after anesthesia did not indicate abnormalities and we found no prominent alterations in other biochemical parameters during or after anesthesia.


Key words: Anesthesia, Halothane, Sheep, Calves, Clinical pathology.