1996-2019. All Rights Reserved. Online Journal of Veterinary Research. You may not store these
pages in any form except for your own personal use. All other usage or
distribution is illegal under international copyright treaties. Permission
to use any of these pages in any other way besides the before mentioned must be
gained in writing from the publisher. This article is
exclusively copyrighted in its entirety to OJVR. This article may be copied
once but may not be, reproduced or re-transmitted without the express
permission of the editors. This journal satisfies the refereeing requirements
(DEST) for the Higher Education Research Data Collection (Australia). Linking:
To link to this page or any pages linking to this page you must link directly
to this page only here rather than put up your own page
OJVRTM
Online Journal of
Veterinary Research©
Volume
21(4):184-188, 2017.
Polymorphism in growth hormone gene of
Khuzestan buffalo.
Armin Saed 1, Hamid Reza Seyedabadi2*, Cyrus Eydivandi1
1Department
of animal science, College of Agriculture, Behbahan
Branch, Islamic Azad University, Khoozestan, 2Animal
Science Research Institute of IRAN (ASRI), Agricultural Research Education and
Extension Organization (AREEO),Karaj,Iran
ABSTRACT
Saed A, Seyedabadi HR, Eydivandi C., Polymorphism in growth hormone gene of
Khuzestan buffalo by PCR-RFLP. Onl
J Vet Res., 21(4):184-188, 2017. Authors report polymorphism in growth hormone gene
by testing 200 Khuzestan buffalo blood samples selected by herd book and phenotypic
standard for the breed. Restriction patterns were
visualized by agarose gel electrophoresis and ethidium bromide staining.
Individual PCR-RFLP fragment sizes for the gene were determined by visualizing
band patterns under ultraviolet light. A 428 bp fragment of growth
hormone gene spanning over 4th exon, 4th intron and 5th exon was amplified and
digested with AluI restriction endonuclease to
identify polymorphism at this locus. Digestion rendered 2 types of genotypes. Distribution
of genotypes and allele frequency by Hardy-Weinberg equation were LL=194 (97%) and LV=6 (3%). Most buffalo were homozygous (LL) and less
heterozygous for the valine allele compared with homozygous leucine allele. Results suggested that diversity of GH gene in
Khuzestan buffalo was very low and showed no polymorphisms. Production traits could not be associated with buffalo growth hormone genotypes due to their
monomorphic haplotype.
Keywords: Growth hormone gene, Polymorphism, PCR-RFLP,
Khuzestan buffalo.