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OJVRTM
Online Journal of Veterinary Research©
Volume 19(3): 169-175, 2015.
Molecular serology of E. coli O157 in ground beef
samples.
Ladan Mansouri-najand DVM PhD, Morteza
Seyfadini DVM,
Mojgan Manzari.
School of Veterinary Medicine, Shahid Bahonar University of
Kerman, Iran
ABSTRACT
Mansouri-Najand L, Seyfadini
M, Manzari
M., Molecular serology of E. coli O157 in ground beef samples, Onl J Vet Res., Volume 19(3): 169-175, 2015. Shiga toxigenic
E. coli (STEC) causes haemolytic uremic syndrome (HUS), haemorrhagic colitis
(HS) and thrombocytopenic purpura in humans.
Ground beef samples were enriched in modified tryptose
soy broth, plated on
sorbitol CT-SMAC and CHROM agar samples after IMViC
test and serotyped as E. coli O157. From 100 samples, 4 isolates were
confirmed by PCR as E. coli O157.
Among the positive samples, one sample had stx1 and stx2, another
one had stx1, eae and hyl A. one was positive for stx1 and the last
one had just stx2. Cattle are thought to be reservoir for E. coli
O157 so ground beef could be considered a risk for food born –STEC
contamination and hazard for human health. A minute number of E. coli
O157 H7 strains were isolated from animal samples but contamination during slaughter,
transport and distribution is also likely.
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