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OJVRTM
Online Journal of
Veterinary Research©
Volume 16 (4):224-240, 2012, Redacted 2017.
Identification
of Staphylococcus spp in bovine milk and teat
skin by PCR–RFLP of gap and groEL
genes.
Narjes
Ghafari DVM, Mohammad Tabatabaei DVM,
Zahra Esmailnezhad DVM
Department of Pathobiology, School of Veterinary Medicine,
Shiraz University, Shiraz, Iran
ABSTRACT
Ghafari N, Tabatabaei M, Esmailnezhad
Z., Identification of Staphylococcus spp in bovine milk and teat skin by PCR–RFLP of gap and groEL genes Online J Vet Res., 16 (4):224-240, 2012. Use of nucleic
acid targets provides an alternative technique for accurate identification of Staphylococcus
species. The groEL
gene has well-conserved DNA sequences but with sufficient sequence variations
that allow species-specific identification. The gap gene commonly
has been considered a constitutive housekeeping gene and encodes a 42-kDa
transferrin-binding protein located within the cell wall of the staphylococci.
We compared phenotypic and genotypic identification methods used for Staphylococcus
spp. isolated from mastitic bovine milk and teat
skin. PCR-RFLP of both the groEL and gap
genes might be useful in reference laboratories for characterization of strains
that could not be assigned to a species on the routine microbiology
laboratories.
Keywords: coagulase negative staphylococci, groEL, gap, PCR-RFLP
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