Volume 24 (3):175-190, 2020.
Formulation of Camellia sinensis phytosome encapsulated Diclofenac and effect on analgesia and inflammation in mice.
Hammad Mohammad Allawi and Mohanad A. Al-Bayati
College of Veterinary Medicine, University of Baghdad, Iraq.
Mohammad Allawi H, Al-Bayati MA., Formulation of Camellia sinensis phytosome encapsulated Diclofenac and effect on analgesia and inflammation in mice, Onl J Vet Res., 24 (3):175-190, 2020. Nanotechnology can deliver drugs in a form which can reduce dose and adverse effects. Green tea (GT) Camellia sinensis can bind to phosphatidylcholine phytosome to encapsulate Diclofenac. We report detailed manufacture of GT phytosome encapsulated diclofenac and its effects on analgesia and inflammation in Balb-c mice. We obtained 25.2 ± 2.7% per 15g Green tea (GT) through 8 methanol soxhlet extractions. The phytosome was constructed by thin-film Bangham method with phosphatidylcholine carrier and GT outer layer. Diflonac was dispersed and encapsulated in the inner core. Phytosomes measured 48.1 - 96.3 nm with GT entrapment efficiency of 88.5 and Diflonac 93.3%. Groups of 15 male balb-c mice were then given phytosome with 1, 3, 5, 10 and 15 mg diclofenac with or without GT. Nociceptive analgesia and anti-inflammatory effects were determined. Licking and jumping was assessed 55% with diflonac but 35% with GT diflonac phytosome, ID50 was 0.94 and 0.83, abdominal constrictions and stretching of hind limb 18% and 14% with ID50, 0.69 and 0.57, paw-licking 88% and 63% with ID50, 0.70 and 0.63, respectively. Carrageen-induced paw edema decreased 10% and 14% with ID50, 0.74 and 0.71.
Key words: green tea, phytosome, diclofenac, mice.