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OJVRTM
Online Journal of
Veterinary Research©
Volume 24 (3):175-190, 2020.
Formulation
of Camellia
sinensis phytosome
encapsulated Diclofenac and effect on analgesia and inflammation in mice.
Hammad Mohammad Allawi and Mohanad A. Al-Bayati
College of Veterinary Medicine,
University of Baghdad, Iraq.
ABSTRACT
Mohammad Allawi H, Al-Bayati
MA., Formulation of Camellia
sinensis
phytosome encapsulated Diclofenac and effect on analgesia and
inflammation in mice, Onl J Vet Res., 24 (3):175-190, 2020. Nanotechnology can deliver
drugs in a form which can reduce dose and adverse effects. Green tea (GT) Camellia sinensis can bind to phosphatidylcholine phytosome to encapsulate Diclofenac. We report detailed
manufacture of GT phytosome encapsulated diclofenac and
its effects on analgesia and inflammation in Balb-c
mice. We obtained 25.2 ± 2.7% per 15g Green tea (GT) through 8 methanol soxhlet extractions. The phytosome
was constructed by thin-film Bangham method with phosphatidylcholine carrier and GT outer
layer. Diflonac was dispersed and encapsulated in the
inner core. Phytosomes measured 48.1 - 96.3 nm with GT
entrapment efficiency of 88.5 and Diflonac 93.3%. Groups
of 15 male balb-c mice were then given phytosome with 1, 3, 5, 10 and 15 mg diclofenac with or without GT. Nociceptive
analgesia and anti-inflammatory effects were determined. Licking
and jumping was assessed 55% with diflonac but 35%
with GT diflonac phytosome,
ID50 was 0.94 and 0.83, abdominal constrictions and stretching of hind
limb 18% and 14% with ID50, 0.69 and 0.57, paw-licking 88%
and 63% with ID50, 0.70 and 0.63,
respectively. Carrageen-induced
paw edema decreased 10% and 14% with ID50,
0.74 and 0.71.
Key words: green tea, phytosome, diclofenac, mice.
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