2020-2032. All Rights Reserved. Online Journal of Veterinary Research. You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking: To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page.


OJVRTM

Online Journal of Veterinary Research

Volume 24 (3):175-190, 2020.


Formulation of Camellia sinensis phytosome encapsulated Diclofenac and effect on analgesia and inflammation in mice.

 

Hammad Mohammad Allawi and Mohanad A. Al-Bayati

 

College of Veterinary Medicine, University of Baghdad, Iraq.

 

ABSTRACT

 

Mohammad Allawi H, Al-Bayati MA., Formulation of Camellia sinensis phytosome encapsulated Diclofenac and effect on analgesia and inflammation in mice, Onl J Vet Res., 24 (3):175-190, 2020. Nanotechnology can deliver drugs in a form which can reduce dose and adverse effects. Green tea (GT) Camellia sinensis can bind to phosphatidylcholine phytosome to encapsulate Diclofenac. We report detailed manufacture of GT phytosome encapsulated diclofenac and its effects on analgesia and inflammation in Balb-c mice. We obtained 25.2 2.7% per 15g Green tea (GT) through 8 methanol soxhlet extractions. The phytosome was constructed by thin-film Bangham method with phosphatidylcholine carrier and GT outer layer. Diflonac was dispersed and encapsulated in the inner core. Phytosomes measured 48.1 - 96.3 nm with GT entrapment efficiency of 88.5 and Diflonac 93.3%. Groups of 15 male balb-c mice were then given phytosome with 1, 3, 5, 10 and 15 mg diclofenac with or without GT. Nociceptive analgesia and anti-inflammatory effects were determined. Licking and jumping was assessed 55% with diflonac but 35% with GT diflonac phytosome, ID50 was 0.94 and 0.83, abdominal constrictions and stretching of hind limb 18% and 14% with ID50, 0.69 and 0.57, paw-licking 88% and 63% with ID50, 0.70 and 0.63, respectively. Carrageen-induced paw edema decreased 10% and 14% with ID50, 0.74 and 0.71.

 

Key words: green tea, phytosome, diclofenac, mice.


MAIN

 

FULL-TEXT (SUBSCRIBE OR PURCHASE TITLE)