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Online Journal of Veterinary Research©
Volume 23 (6):522-543, 2019.
Effect of Green tea (Camellia sinensis) on acrylamide induced cardiac toxicity in adult Wistar rats.
Hind Abood Mahdi and Baraa Najim Al-Okialy
Department of Physiology, Biochemistry and Pharmacology, College of Veterinary Medicine /University of Baghdad, Iraq.
Mahdi HA, Al-Okialy BN., Effect of Green tea (Camellia sinensis) on acrylamide induced cardiac toxicity in adult Wistar rats, Onl J Vet Res., 23 (6):522-543, 2019. We report effect of oral alcoholic extract of green tea Camellia sinensis on cardiac toxicity induced by acrylamide (ACR) in rats. Four groups of 5 female Wister rats each were intubated 1mg/kg acrylamide (1) with (2) or without (3) 200mg/kg alcoholic extract of green tea (GT) daily for 35 days. Controls were intubated distilled water (4). Fasting cardiac puncture blood samples were collected at 0, 21 and 35 days under anesthesia for serum catalase, peroxy-nitrite radicals and troponin–I (cTn-I). Cardiac tissue was excised for malondialdehyde (MDA), reduced glutathione (GSH) and blood for DNA fragmentation. In rats given ACR alone, we detected decreased catalase and GSH but increased cTn-I, peroxynitrite radicals and MDA in cardiac tissue In those given ACR we found MDA with DNA damage in leukocytes compared with other treatments. In rats given ACR by comet assay we found increased DNA fragmentation, comet length, head diameter, tail length (D), DNA% in tail (E) and tail moment (F). In rats given green tea extract with or without ACR results were similar to water controls. Phenols in green tea may ameliorate oxidant/antioxidant status of cardiac tissue in rats exposed to ACR.
Key-Words : acrylamide , green tea, cardiac tissue, DNA fragment, raw data provided after references.