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OJVRTM

 

Online Journal of Veterinary Research©

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X

 

Volume 28(5): 294-301, 2024.


Antibiotic resistance and plasmid Vibrio para-hemolyticus from cockles.

 

Bilung LM1, Radu S2, Bahaman AR3, Rahim RA4, Napis S4, Apun K5,

Queen CY6, Marlina K2,  Tanil GB2, Nishibuchi M7.

 

1Department(s) Biotechnology, Faculties Engineering and Science, University Tunku Abdul Rahman, 2Food Science, Food Science and Technology, 3Veterinary Medicine, 4Cell and Molecular Biology, Biotechnology and Biomolecular Sciences,  6Biomedical Science, Health Sciences, University Putra Malaysia,  5Resource Biotechnology, Resource Science and Technology, Universiti Malaysia Sarawak, Malaysia and 7Center for Southeast Asian Studies, Kyoto University, Yoshida, Sakyo-ku, Kyoto,  Japan.

 

ABSTRACT

 

Bilung LM, Radu S, Bahaman AR, Rahim RA, Napis S, Apun K, Queen CY, Marlina K,  Tanil GB, Nishibuchi M., Antibiotic resistance and plasmid Vibrio para-hemolyticus from cockles, Onl J Vet Res., 28(5): 294-301, 2024. We report incidence of V. para-hemolyticus in 100 cockles from Malaysian peninsula. By PCR we found 62 with Vibrio para-hemolyticus screened for tdh and trh gene resistance 0.58 to 0.94 with 89% hosting one to three 2.7 to 54kb sized plasmids. We tested carbenicillin, ceftriaxone, cephalothin, chloramphenicol, clindamycin, doxycycline, imipenem,  nalidixic acid, netilmicin, nitrofurantoine), norfloxacin, oflaxacin, rifampin, streptomycin, sulfamethixazole, teicoplanin and tobramycin finding 100% resistance to nearly all except ceftriaxone (85%), imipenem (79%), netilmicin (74%), nitrofurantoine (63%), doxycycline (60%), nalidixic acid (60%), chloramphenicol (24%) but norfloxacin only 2%. All strains of V. para-hemolyticus plasmids from cockles showed multiple antibiotic resistance (MAR) but none were predictive of antibiotic susceptibility.

 

Key Words: Vibrio para-hemolyticus, cockles, antibiotics, susceptibility.


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