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OJVRTM

Online Journal of Veterinary Research ©

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X

Volume 28 (2): 77-89, 2024.

In Silico docking human glut5 protein intestinal transport.

GV Padmavathi¹, D Saralakumari¹, P Nataraj Sekhar²

¹Department of Biochemistry, Sri Krishnadevaraya University, Anantapur,²Katholike University (Leuven), Belgium.

ABSTRACT

Padmavathi GV, Saralakumari D, Nataraj Sekhar P., In Silico docking human glut5 protein intestinal transport, Onl J Vet Res., 28 (2): 77-89, 2024. GLUT5 in enterocytes facilitates fructose transport from intestinal lumen into blood. We constructed In silico 3D GLUT5 protein model interaction between sweeteners and inhibitors by crystal structure of glutaminase glucosamine 6-phosphate synthase. Model was designed by pico-second molecular dynamics simulation and minimization assessed by ERRAT, WHATCHECK and PROCHECK. Docking studies between sweeteners and inhibitors suggested fructose had most affinity but ingliforib exhibited most interaction with GLUT5. Our findings suggested Asn 94, Lys156, Asn 157 and Gln 178 were required residues for hydrogen bond binding with ligands.

Key words: GLUT5, homology modelling, Molecular Dynamics, Docking.

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