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OJVRTM

Online Journal of Veterinary Research©

Volume (9):926-934, 2019.


Prevalence and identification of Giardia duodenalis in goats (Iraq) by standard and PCR tests.

 

Hajer Sabah Nooriand and Haider Mohammed Ali Al-Rubaie.

 

Department of Parasitology, College of Veterinary Medicine, University of Baghdad , Baghdad ,Iraq.

 

ABSTRACT

 

Noori HS,Al-Rubaie HMA., Prevalence and identification of Giardia duodenalis in goats (Iraq) by standard and PCR tests., Onl J Vet Res., (9):915-923, 2019. Authors report prevalence of Giardia duodenalis in 180 goats in Iraq by direct wet smear, Lugolís iodine and Giemsa stains, flotation or PCR. The Triose phosphate isomerase gene was used to determine Giardia duodenalis by PCR in feces of 100 goats, bands fractionated at 550 bp visualized by ethidium bromide stain in NCBI under LC 477218 and LC 477219. The genotypes were highly related (99-100%) to other known Giardia from most countries. Our PCR used genotyping with ss-rRNA and tpi primers specific for G. duodenalis from Assemblage E. Prevalence by traditional methods was ~31% (55/180), and by PCR ~15% (15/100). Age and gender were not related with infection rate. By PCR sequencing, we found 99-100% identity with Giardia duodenalis.

 

Key-Words: Giardiasis, goats, Iraq.


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