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OJVRTM
Online Journal of Veterinary Research©
Volume (9):926-934, 2019.
Prevalence and identification of Giardia duodenalis
in goats (Iraq) by standard and PCR tests.
Hajer Sabah Nooriand and Haider Mohammed Ali Al-Rubaie.
Department of Parasitology, College of
Veterinary Medicine, University of Baghdad , Baghdad
,Iraq.
ABSTRACT
Noori HS, Al-Rubaie
HMA., Prevalence and identification of Giardia
duodenalis in goats (Iraq) by standard and PCR
tests., Onl J Vet Res., (9):915-923, 2019. Authors report prevalence of Giardia duodenalis in 180 goats in Iraq by direct wet smear, Lugol’s iodine and Giemsa stains, flotation or PCR. The Triose phosphate isomerase gene was used
to determine Giardia duodenalis by PCR in
feces of 100 goats, bands fractionated at 550 bp
visualized by ethidium bromide stain in NCBI under LC 477218 and LC 477219. The
genotypes were highly related (99-100%) to other known Giardia from most countries. Our PCR used genotyping with ss-rRNA and tpi primers specific
for G. duodenalis from Assemblage E. Prevalence
by traditional methods was ~31% (55/180), and by PCR ~15% (15/100). Age and
gender were not related with infection rate. By PCR sequencing, we found 99-100%
identity with Giardia duodenalis.
Key-Words:
Giardiasis, goats, Iraq.
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