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Online Journal of Veterinary Research©
Volume 22 (3): 165-173, 2018.
Effect of vitamin B6 on gentamicin-induced nephrotoxicity in Sprague-Dawley rats.
A. Derakhshanfar (Phd-DVM); A. Bidadkosh (DVM) and M. H. Imanian (DVM).
Department of Pathology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
Derakhshanfar A, Bidadkosh A, Imanian MH., Effect of vitamin B6 on gentamicin-induced nephrotoxicity in Sprague-Dawley rats, Onl J Vet Res., 22 (3): 165-173, 2018. Effect of low and high dose supplement of vitamin B6 on gentamicin-induced nephrotoxicity in Sprague Dawley rats is described. It has been postulated that there is a correlation between pyridoxal 5'-phosphate (PLP) level and nephrotoxicity. Groups of 10 male Sprague-dawley rats each were injected intramuscular (IM) 80mg/kg gentamicin, 200mg/Kg vitamin B6, and 80mg/Kg gentamicin with 100mg/kg or 200mg/kg vitamin B6 daily for 10 days. Controls were injected 5 ml distilled water. After treatments, serum creatinine, sodium, potassium, renal gentamicin and PLP concentrations were determined along with microscopy of kidneys. Compared with controls, gentamicin increased in renal tissue whereas creatinine clearance, sodium, potassium and kidney PLP declined. 200 mg/kg vitamin B6 with gentamicin reduced renal gentamicin concentration, increased creatinine clearance and reduced serum sodium and potassium. Microscopy revealed minimal changes in renal tissue of rats co-administed B6 with gentamycin. Changes described above were significant (P < 0.05). Results suggested that B6 at high doses may protect kidney tissue against gentamycin toxicity in Sprague-Dawley rats.
Keywords: Gentamicin, Nephrotoxicity, Vitamin B6, Pyridoxal 5-phosphate, Rat.