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OJVRTM
Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 26 (4): 280-288,
2022.
Polymorphism of GDF9
gene and its association with litter size in goats
Elham Javdan, Jamal Fayazi, Mohammad Taghi Baigi Nasiri, Saleh Tabatabaei Vakili.
Department of Animal
Science, Ramin University of Agriculture and Natural
Resources, Ahwaz, Iran
ABSTRACT
Javdan E, Fayazi
J, Baigi Nasiri MT, Vakili ST., Polymorphism
of GDF9 gene and its association with litter size in goats, Onl
J Vet Res., 26
(4): 280-288, 2022. We report association of
differentiation factor 9 (GDF9) gene with litter size
in goats. The polymorphism of exon 2 of GDF9 gene was detected by PCR-SSCP in
two goat breeds with different prolificacy. 121 genomic DNA samples were taken from Najdi and Lori-Bakhtiari goats.
After extraction 295 bp fragment of exon 2 of GDF9
gene was amplified by PCR with specific primers and assayed by single stranded
conformation polymorphism. Frequencies of genotypes AA, AB
and BB were 0.37, 0.423 and 0.207 in Najdi goats and 0.586, 0.173 and 0.241 in Lori-Bakhtiari breeds, respectively. Heterozygosity in Najdi goats was 0.424 and 0.172 in Lori-Bakhtiari.
KEY-WORDS : Goat Prolificacy, Growth
differentiation factor 9 gene, PCR-SSCP.
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