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OJVRTM
Online
Journal of Veterinary Research©
Volume 19(4): 282-289, 2015.
Effect of gonadotropin and frozen-thawed culture media on oocyte and
zygote production
S-M Mirshamsi1, H Karami-Shabankareh1, J Habibizad2
1IVF & ET Laboratory,
Department of Animal Science, Razi University,
Kermanshah, Iran;2Department of Animal Science, Faculty of
Agriculture, Yasouj University, Yasouj,
Iran
ABSTRACT
Mirshamsi SM, Karami-Shabankareh H, Habibizad J., Effect of gonadotropin and frozen-thawed
culture media on oocyte and zygote production, Onl J
Vet Res., 19(4): 282-289, 2015. Oocytes taken
from non-breeding ovine ovaries were cultured in maturation medium supplemented
with 0.02 IU/mL pituitary, 0.075 IU/mL recombinant and/or 10 IU/mL chorionic
gonadotropins. In vitro matured
oocytes were transferred into fresh medium as controls, frozen-thawed In vitro fertilization (FT-IVF) and
frozen-thawed In vitro culture
(FT-IVC) media. Zygotes from FT-IVF medium were cultured again in FT-IVC medium
(FT-IVFC). There were no differences in blastocysts using pituitary (21.1%),
recombinant (17.3%) or chorionic (20.5%) gonadotropins or fresh (42.0%), FT-IVF
(44.3%), FT-IVC (42.8%) or FT-IVFC (48.2%) media. Results suggest that
non-pituitary gonadotropins and frozen-thawed culture media could support
embryonic development.
Key words:
immature oocytes, maturation medium, zygotes, culture medium, brilliant cresyl blue.
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