1996-2019. All Rights
Reserved. Online Journal of Veterinary Research. You may not store these pages in any form except
for your own personal use. All other usage or distribution is illegal under
international copyright treaties. Permission to use any
of these pages in any other way besides the before mentioned must be gained in
writing from the publisher. This article is exclusively copyrighted
in its entirety to OJVR. This article may be copied once but may not be,
reproduced or re-transmitted without the express permission of the editors.
This journal satisfies the refereeing requirements (DEST) for the Higher Education
Research Data Collection (Australia). Linking: To link to this page or any
pages linking to this page you must link directly to this page only here rather
than put up your own page.
OJVRTM
Online Journal of
Veterinary Research©
Volume
21(1):8-14, 2017.
Effect of Fraxinus excelsior on dermal collagen during wound healing in
adult Oryctolagus caniculus rabbits.
Sahel Motaghi,
PhD1*, Mohammad Sadeghi, DVM2, Sajad Seyedin, DVM2,
Reza Kheirandish, PhD3, Mohammad Naser Nazem, PhD1,
Mansour Mirtadzadini,
PhD 4, Gholam Reza Sepehri,
PhD5, Mohammad Mehdi Oloumi, DVSc6
Department(s)
of 1Basic Sciences, 3Pathobiology, 6Clinical
Sciences, 2Faculty of Veterinary Medicine, 4Biology,
Faculty of Science Shahid Bahonar
University of Kerman, 5Neuroscience Research Center, Kerman
University of Medical Science, Kerman, Iran. *Correspondence:
sahelmotaghi@gmail.com
ABSTRACT
Motaghi
S, Sadeghi M, Seyedin S, Kheirandish R, Nazem MN,
Mirtadzadini, M, Sepehri
GR, Oloumi MM., Effect of Fraxinus
excelsior on dermal collagen during wound healing in adult Oryctolagus
caniculus rabbits, Onl J
Vet Res., 21(1):8-14,
2017. Effect of Fraxinus
excelsior alcoholic extract on thickness of dermal papillary collagen
during healing in 4 male and 3 female adult Oryctolagus
caniculus rabbits is described. Under anesthesia,
4 wounds were created on each rabbit and treated daily with 0.2g eucerin as negative controls, phenytoin 1% positive
controls and Fraxinus excelsior 10%,
and 20% in eucerin-based creams for 20 days. At
euthanasia on day 21, skin sections were taken and
stained with Masson's trichrome to
measure thickness of collagen bundles and
hematoxylin-eosin
(H&E) for histopathology. On Masson's
trichrome, average diameter of 10 collagen bundles in
every 10 fields were measured by digital lens Dino-eye, AM-7023, 5Mp and
H&E sections for inflammatory cells and angiogenesis. Results suggested that
Fraxinus excelsior 10% cream increased (P < 0.01) collagen bundle thickness
in the papillary layer compared with eucerin but had
no effect on collagen in the reticular layer. As expected, phenytoin increased
(P < 0.01) collagen thickness in both reticular and papillary layers. .
Keywords: Fraxinus excelsior, wound
healing, collagen, rabbit.
FULL-TEXT(SUBSCRIPTION
OR PURCHASE TITLE $25USD)