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OJVRTM

 Online Journal of Veterinary Research©

Volume 18(3): 293-302, 2014. Redacted 2017.


FOXE1: Methylation and gene expression in canine osteosarcoma cell culture.

 

Araújo RF1, Bersano PRO1, Ferrasi AC2, Montoya LM1,3,Da Silva GN4, Feo HB1, Rocha NS1

 

1Department(s) of Veterinary Clinics and 2Pathology, School of Veterinary Medicine and Animal Science, Universidade do Estado de São Paulo, Botucatu, São Paulo, Brazil, 3Veterinary Pathology Research Group, Faculty of Agricultural Sciences, Universidad de Caldas, Manizales, Colombia, 4Department of Clinical Analysis. Pharmacy School, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil

 

ABSTRACT

 

Araújo RF, Bersano PRO, Ferrasi AC, Montoya LM, Da Silva GN, Feo HB, Rocha NS., FOXE1: Methylation and gene expression in canine osteosarcoma cell culture, Onl J Vet Res., 18(3): 293-302, 2014. Gene expression profiles in a canine osteosarcoma (OSA) cell culture could identify chemotherapeutic targets and sort useful from useless prognostic groups. This could allow for more personalized treatment of OSA based on an individual’s tumor expression profile. An experimental model of methylation and gene expression in canine osteosarcoma cell culture is described. Blood Samples were taken from 5 dogs with cytological and histo-pathological OSA, and one from normal bone. OSA primary cell culture was isolated and characterized. For methylation and FOXE1 expression analysis, DNA of 5x106 osteosarcoma cells was used. Results showed that about 80% OSA cell cultures sequences were methylated and 20% un-methylated. FOXE1 expression was not demonstrated in un-methylated cell culture or normal bone. The findings suggest that FOXE1 gene may be silenced in OSA cells but its absence in osteoblast cells suggests that it is not involved in the carcinogenesis of canine osteosarcoma.

 

Key words: Immunocytochemical, epigenetic, osteoblast, FOXE1 gene expression.


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