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OJVRTM
Online Journal of Veterinary Research©
Volume 17 (11):649-654, 2013

Suspension culture of fowlpox virus  in Theileria annulata-infected cell line

 

Karimiyan A1, Namavari M2* , Mansourian M2,  Rahimian A1 , Tahamtan Y1

 

1Islamic Azad University of Jahrom, Jahrom, 2Razi Vaccine and Serum Research Institute, Shiraz, Iran

 

ABSTRACT

 

Karimiyan A, Namavari M, Mansourian M,  Rahimian A , Tahamtan Y., Suspension culture of fowlpox virus  in Theileria annulata-infected cell line, Onl J Vet Res., 17 (11):649-654, 2013 Currently fowlpox vaccines are manufactured using embryonated chicken egg which is intrinsically cumbersome and not scalable to provide large quantities. Efficient vaccine production requires growth of large quantities of virus produced with high yield from a safe host system. A suspension cell culture of Theileria annulata-infected lymphoblastoid (TIL) was used as a host cell for fowlpox virus. Adaptation and proliferation of purified virus was carried out on the cell line and TCID50 was calculated. Results suggested that this cell line could be tested as an alternative to eggs in fowlpox vaccine production

 

Keywords: fowlpox virus , Theileria annulata-infected lymphoblastoid , suspension culture.

 

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