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OJVRTM

 

Online Journal of Veterinary Research

(Including Medical and Laboratory Research)

Established 1994

ISSN 1328-925X

 

Volume 28(5): 263-269, 2024.


Single dilution ELISA for foot and mouth disease serotype viruses.

Kumar B, Manuja A, Kumar A

Central Institute for Research on Buffaloes, Hisar Veterinary Microbiology, CCS Haryana Agricultural University, Hisar India

 

ABSTRACT

 

Kumar B, Manuja A, Kumar A., Single dilution ELISA for foot and mouth disease serotype viruses, Onl J Vet Res., 28(5): 263-269, 2024. Solid (SP) and liquid phases (LP) single dilution ELISA were developed for foot and mouth disease virus antibodies of serotypes O, Asia 1 and subtype A22. Antibody titers of 80 cattle serum before and 21 days after vaccination with polyvalent vaccine were determined by multi-dilution ELISA for inhibition by 2 fold serum dilutions of 1:10 to 1:80. Maximum correlation was found at 1:40 dilution for all serotypes except for Asia 1 LP ELISA at 1:80 dilution. Serum antibody titres of 20 cattle serum samples estimated by multidilution and single dilution ELISA revealed no significant difference between the antibody titers.

 

Keywords: Foot and Mouth disease, single dilution ELISA, percent inhibition.


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