©1996-2015 All Rights Reserved. Online Journal of
Veterinary Research . You may not store these pages in any form except for your
own personal use. All other usage or distribution is illegal under
international copyright treaties. Permission to use any of these pages in any
other way besides the before mentioned must be gained in writing from the
publisher. This article is exclusively copyrighted in its entirety to OJVR.
This article may be copied once but may not be, reproduced or re-transmitted
without the express permission of the editors. This journal satisfies the
refereeing requirements (DEST) for the Higher Education Research Data
Collection. Linking:To link to this page or any pages linking to this page you
must link directly to this page only here rather than put up your own page.
OJVRTM
Online Journal of Veterinary Research©
Volume 19(2):
47-65, 2015.
Effect of diet on follicle
size and expression of FSH receptor variants in ewes with progesterone
implants.
Amir Hossein Asgari Safdar1* and Vahid
Ighani1
1Department
of Animal Sciences, Faculty of Agriculture and Natural Resources, Science and Research Branch, Islamic Azad University,
Tehran, Iran.
ABSTRACT
Asgari Safdar AH, IghanimV., Effect of diet on follicle
size and expression of FSH receptor variants in ewes with progesterone
implants, Onl J Vet Res., 19(2): 47-65, 2015. Flushing and expression of follicle stimulating hormone (FSH)
receptor variants in Afshari Ewes fed different diets
are described. Ninety three 1yr old Afshari ewes were
implanted with a progesterone releasing devices (CIDR) to synchronize estrus
and follicular development. Ewes were fed 6 combination diets with alfalfa,
with or without prairie hay, or block and rolled corn supplements for 2 weeks.
CIDR were removed and aspirated follicles were categorized medium sized 4 to 6
or larger > 6 mm. Total RNA was extracted from granulosa cells and reverse
transcribed by qPCR of the resulting cDNA with primer sets for each variant of
FSHR and LH receptor. Expression of FSHR-1 was different between medium and
large follicles (P < 0.01) and ewes fed alfalfa hay (P<0.05). Overall
mean expression of FSHR-3 was greater than FSHR-1 or FSHR-2 (P<0.01), but
there was no difference in these values between medium or larger follicles, or
between diets. Concentrations of
estradiol in follicular fluid were not affected by diet, follicle size nor expression of lutenizing hormone receptor (LHR), suggesting that
follicles developed equally. Live weights were different (P < 0.01) between
diets but there were no differences in any group between non-esterified fatty
acids concentrations, mean body condition (3.0) or lambing rates. Results suggested that FSHR-1 was involved in
later stages of follicular development, and possibly in follicle rescue. FSHR-3
was the most highly expressed FSHR and could be essential for antral follicle
development. The findings show that a flushing response was not achieved in
this study. Research is needed to determine how the initial energy status in
ewes increases ovulation rate after energy supplementation (i.e. the flushing
response).
Key Words:
Flushing, Follicle Stimulating Hormone (FSH), Variants, Afshari
ewes.
FULL-TEXT(SUBSCRIBERS) OR PURCHASE
ARTICLE $25USD