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OJVRTM

Online Journal of Veterinary Research© 

Volume 19(8): 491-496, 2015.

 

Effect of fluoride on cariogenic Streptococcus mutans and Lactobacillus counts

in children as determined by quantitative real time-PCR.

 

Jahanimoghadam F1, Bazrafshani MR2, Hali H3, Hosseini F4.

 

1Kerman Oral & Dental Research Center, Department of Pediatric Dentistry, Faculty of Dentistry, 2Department of Medical Genetics, Afzalipour School of Medicine and Physiology Research center, Kerman University of Medical Sciences, 3Department of Pediatric Dentistry, Faculty of Dentistry, Mazandaran University of Medical Sciences, Sari, 4Medical Genetics Laboratory, Kerman, Iran.

 

ABSTRACT

 

Jahanimoghadam F, Bazrafshani MR, Hali H, Hosseini F., Effect of fluoride on cariogenic Streptococcus mutans and Lactobacillus counts in children as determined by quantitative real time-PCR, Onl J Vet Res., 19(8): 491-496, 2015. Effect of sodium fluoride 2% (NaF) and Acidulated Phosphate Fluoride gels (APF-gel) on cariogenic Streptococcus mutans and Lactobacillus counts were compared by quantitative real-time PCR in children. Fluoride was applied on primary and permanent teeth as an APF gel of 1.23% fluoride ion, 0.98% phosphoric acid with pH= 3.5 in 22 children or as NaF  2% gel, 0.9% fluoride ion with pH of 6.5-7.5 to another group of 22 children. Controls consisted of before treatment samples. Results suggested that APF-gel treatment reduced (P <0.05) bacteria compared with NaF-gel. QRT-PCR is a reliable rapid method for identification of cariogenic S. mutans and Lactobacili

 

Keywords: Dental caries, Streptococcus mutans, Lactobacillus, Quantitative Real Time PCR, Fluoride.

 

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