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Online Journal of Veterinary Research


Volume 26 (7): 519-528, 2022.

Inhibition of complement C5a Cteneocephalides felis flea allergen dermatitis in mice


Ku Nurul Atiqah KA DVM, Watanabe M DVM PhD, Farhana BN Bsc , Norhaifa G Bsc,

Hazwani KN Bsc, Nashreq KN Bsc and Hezmee MNM DVM PhD


Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia 43400 Serdang, Selangor, Malaysia.


Nurul Atiqah KA, Watanabe M, Farhana BN, Norhaifa G, Hazwani KN, Nashreq KN, Hezmee MNM, Inhibition of complement C5a Cteneocephalides felis flea allergen dermatitis in mice, Onl J Vet Res., F26 (7): 519-528, 2022. Flea allergy dermatitis (FAD) in dogs is mostly induced by complement C5a from Ctenocephalides felis. We report effect of complement inhibitor to inhibit activation of C5a receptor allergic dermatitis in BALB/C mice. We compared results with prednisone and assessed effects on tissue, hematology and biochemical values. Four groups of 5 male and female mice each were injected 100g/mouse flea antigen intramusculary and intradermally and then injected daily for 3 days 0.1mg/kg complement C5 inhibitor (1) or 0.1mg/kg prednisolone (2). Controls were given only flea antigen (3) or anti-histamine (4). We found no effect on leucocytes, lymphocyte, neutrophils or eosinophils but observed spongiosis and inflammation in epidermis. Dermis of controls given only antigen measured ~48m, complement inhibitor 42m, antihistamine 39m and prednisone 26m representing a difference of 46%. However only controls injected flea antigen without treatment developed hyperplasia of epidermis (P < 0.05)


Key Words: Ctenocephalides, complement C5, prednisone, canine, mice, dermatitis.