Effect of oral ferrous sulphate on subchronic cadmium
chloride toxicity in adult male albino rats.
Riyadh K Farhood and Duraid
A Abass.
Department of Physiology, Biochemistry and
Pharmacology, College of Veterinary Medicine – University of Baghdad, Bagdad,
Iraq
ABSTRACT
Farhood RK, Abass DA., Effect of oral ferrous
sulphate on subchronic cadmium chloride toxicity in
adult male albino rats, Onl J Vet Res., 23
(7):707-716, 2019. We report effect of oral ferrous sulphate on subchronic
cadmium toxicity in adult male albino rats. Groups of 7 rats each were gavaged 1.3mg/kg ferrous sulphate (Fs) with or without 1/25
LD50 11mg/kg cadmium chloride (Cd) daily for 45 days. Controls were gavaged 0.1 ml/100g distilled water. At Day 45, serum alanine
(ALT) and aspartame (AST) transaminases, cadmium, iron (Fe+2) and calcium (Ca+2
) ions and haematology were determined.
Compared with controls (P < 0.05) we found ALT increased ~20% in rats
but only ~3-6% in those given Fs with or without Cd and AST ~40% and 18-23%, respectively. White
blood cells (WBC) increased 21-31% in rats given Cd or Fs. However red blood
cells (RBC) declined ~5% in rodents given Cd but increased in those given Fs
with or without Cd (15%). Platelets fell and clotting time increased in rats
given Cd or Fs. Treatments with ferrous sulphide
per se appear to have boosted RBC but
did not ameliorate increased clotting times or reduced platelets in any group. As expected serum cadmium and Fe+2 iron increased in treated rats. There were large falls
in serum Ca+2 (61-78%) in
rats gavaged Fs or Cd. Results suggest
that ferrous sulphide may have prevented rises in
serum cadmium which may have ameliorated toxicity in rats.
Keyword: Cadmium toxicity, ferrous sulphate, serum ALT,
AST, hematology, metals. Raw data provided.