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OJVRTM
Online
Journal of Veterinary Research©
Volume 19(9): 704-708, 2015.
16S rRNA PCR test for Mycoplasma spp
in sheep
Mehrdad Shamsaddini
Bafti (DVM, PhD)1*,
Seyed Ali Pourbakhsh (DVM,
PhD)2, Majid Ezatkhah1 (DVM), Abbas Ashtari
(DVM)2
Razi Vaccine and Serum
Research Institute, 1Kerman branch, 2Karaj
branch, Iran
ABSTRACT
Bafti MS, Pourbakhsh
SA, Ezatkhah M, Ashtari A.,
16S rRNA PCR test for Mycoplasma spp
in sheep, Onl J Vet Res., 19(9):
704-708, 2015. Mycoplasmas
are bacteria without a cell wall which may be pathogenic in humans or animals. Mycoplasma spp. was isolated from milk, conjunctiva,
ear lesions and puncture of joint exudate of 70 suspected sheep
herds. Mycoplasma was detected by culture and PCR. Mycoplasma spp. were isolated from 14.8% of samples by PCR. Results showed
that a swab of an ear lesion could be suitable for isolation of Mycoplasma spp. in sheep.
Key words: Mycoplasma, PCR, 16 rRNA, Sheep.