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OJVRTM
Online Journal of Veterinary
Research©
Volume 23
(10):1044-1050, 2019.
Effect of Bacillus thuringiensis var kurstaki on scanning ultrastructure of Fasciola gigantica flukes.
Mohey A Hassanina, Faiza M El-Assal, Eman H Abdel-Rahman, Ragaa M Khalifa.
aDepartment of Zoonotic Diseases,
National Research Center, Department of Zoology, Faculty of Science , Cairo
university, Egypt.
ABSTRACT
Hassanin MA, El-Assal
FM, Abdel-Rahman EH, Khalifa RM., Effect of Bacillus thuringiensis var kurstaki on scanning
ultrastructure of mature Fasciola gigantica
worms, Onl J Vet Res., 23 (10):1044-1050, 2019. Fasciola
gigantica adult flukes were treated
in vitro with 0.5, 1 and 2 mg Bacillus
thuringiensis var
kurstaki (BTK).
2mg BTK killed flukes by 24 hours with an LC50 of 1.1 mg/ml.
Scanning electron microscopy of adult worms treated with BTK shows upward
shifting of the oral cone, with neck formation due to stretching of worm tissue
and herniation of internal organ through oral suckers. The transverse folds of
the rim of the oral suckers became lobulated, with loss of sensory papillae and
gland pore formation. There were blebs of different sizes and erosions at the
transverse folds of the oral suckers as well as in the mid-body regions. The
blebs increased in size and burst, resulting in spine loss. Our results suggest
that Bacillus thringiensi var
kurstaki, exhibits vermicidal
activity against F. gigantica in vitro.
Keywords: Fasciola gigantica,
Bacillus
thuringiensis, scanning electron microscopy.
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