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Online Journal of Veterinary Research©
Volume 23 (10):1044-1050, 2019.
Effect of Bacillus thuringiensis var kurstaki on scanning ultrastructure of Fasciola gigantica flukes.
Mohey A Hassanina, Faiza M El-Assal, Eman H Abdel-Rahman, Ragaa M Khalifa.
aDepartment of Zoonotic Diseases, National Research Center, Department of Zoology, Faculty of Science , Cairo university, Egypt.
Hassanin MA, El-Assal FM, Abdel-Rahman EH, Khalifa RM., Effect of Bacillus thuringiensis var kurstaki on scanning ultrastructure of mature Fasciola gigantica worms, Onl J Vet Res., 23 (10):1044-1050, 2019. Fasciola gigantica adult flukes were treated in vitro with 0.5, 1 and 2 mg Bacillus thuringiensis var kurstaki (BTK). 2mg BTK killed flukes by 24 hours with an LC50 of 1.1 mg/ml. Scanning electron microscopy of adult worms treated with BTK shows upward shifting of the oral cone, with neck formation due to stretching of worm tissue and herniation of internal organ through oral suckers. The transverse folds of the rim of the oral suckers became lobulated, with loss of sensory papillae and gland pore formation. There were blebs of different sizes and erosions at the transverse folds of the oral suckers as well as in the mid-body regions. The blebs increased in size and burst, resulting in spine loss. Our results suggest that Bacillus thringiensi var kurstaki, exhibits vermicidal activity against F. gigantica in vitro.
Keywords: Fasciola gigantica, Bacillus thuringiensis, scanning electron microscopy.