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OJVRTM

Online Journal of Veterinary Research©

(Including Medical and Laboratory Research)

Established 1994
ISSN 1328-925X

 

26 (11): 802-809, 2022.


Effect of Eugenol supplement on serum hypophysis, thyroid, follicular and reproductive hormones

and lipids in hypothyroid male Wistar rats

 

Nadia K. J. Al-Dawah, Fouad Zeidan Hamzah, Saadia Saleh Mehdy Zieny

 

Department of Physiology, Pharmacology and Chemistry, College of Veterinary Medicine, University of Kufa, Iraq.

 

ABSTRACT

 

Al-Dawah NKJ, Hamzah FZ, Mehdy Zieny SS., Effect of Eugenol supplement on serum hypophysis, thyroid, follicular and reproductive hormones and lipids in hypothyroid male Wistar rats., Onl J Vet Res., 26 (11): 802-809, 2022. Authors describe effects of eugenol plant extract gavage on serum hypophysis, thyroid and testes hormones in hypothyroid rats over 21 days.  To induce hypothyroidism, 50mg/Kg Propylthiouracil in 1ml saline was injected IP daily for 1 month  in 3 groups of 8 adult male 220-250g Wistar rats each. Rodents were then injected 0.1mg/kg thyroxine IP daily (1), gavaged 100mg/kg eugenol (EU) extract (2) or given both (3) Controls were injected saline IP (4). At day 21, rats were bled for serum TSH, T4, T3, FSH, LH, testosterone (TES), triglycerides (TG), low-and high density lipoproteins (LDL, HDL, VLDL) and cholesterol (CH). Compared (P < 0.05 to 0.0001) with  controls, in hypothyroid rats, TSH was lower (<) <~35% and T4 higher (>) >~85%, but >~9% with EU whereas T3 was <55%  with EU <29% and T4 <17%. Serum FSH <56% but with EU or T4 <~5-6% and LH <~56, <28 and <28%, and testosterone <~46, <21 and <7%, respectively. Serum cholesterol was >34% and >10-16% with EU or T4, TG >23% and >~8 with T4, but normal with EU, HDL <53% and <18% with T4, LDL >55% higher and >23-41% with EU or T4. Similarly VLDL was 54, 21 and 32% higher compared with saline controls. Results suggest eugenol prevented reductions in serum T4 and T3, rises in TSH and reductions in testosterone in hypothyroid Wistar rats. However, extraneous T4 injections had similar effect to eugenol.

 

Keywords: Lipid Profile, Hypothyroidism, Eugenol, Thyroxine, Testosterone.


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