©1996-2018. All
Rights Reserved. Online Journal of Veterinary Research .
You may not store these pages in any form except for your own personal use. All
other usage or distribution is illegal under international copyright treaties.
Permission to use any of these pages in any other way besides the before mentioned
must be gained in writing from the publisher. This article is exclusively
copyrighted in its entirety to OJVR.This article may
be copied once but may not be, reproduced or re-transmitted without the express
permission of the editors. This journal satisfies the refereeing requirements
(DEST) for the Higher Education Research Data Collection (Australia). Linking:To link to this page or
any pages linking to this page you must link directly to this page only here
rather than put up your own page.
OJVRTM
Online Journal of Veterinary Research ©
Volume 15 (3): 215-228, 2011. Redacted 2017.
A comparison of IL-1β
and IL-8 mRNA and their respective proteins in equine bronchoalveolar
lavage fluid during recurrent airway obstruction.
Sarli Giuseppe, Peli Angelo, Marrocco
Romina, Zanotti Lucia, Ducci
Alice1, Isani Gloria, Alessandra Scagliarini,
Sassi Francesco, Benazzi Cinzia, Cinotti Stefano, Pietra Marco.
Dipartimento di Scienze Mediche Veterinarie (Alma Mater Studiorum-
Università di Bologna), 1Corpo Sanitario
Veterinario dell’Esercito Italiano, Roma
ABSTRACT
Sarli G, Peli A, Marrocco R, Zanotti L, Ducci A, Isani G, Alessandra S, Sassi F, Benazzi C, Cinotti S, Pietra M., A
comparison of IL-1β and IL-8 mRNA and their respective proteins in equine bronchoalveolar lavage fluid during recurrent airway
obstruction, Onl J Vet Res., 15 (3): 215-228, 2011. The role of cytokines in triggering airway
inflammation during recurrent airway obstruction (RAO) has become the focus of
recent investigations. The majority of published papers are principally
concerned with cytokine mRNA while there are fewer papers which deal with their
respective proteins alone or their correlation with mRNA in the same trial. In
the present study IL-1β and IL-8 mRNAs and their respective proteins were quantified by Real Time RT-PCR
and western blot and related to bronchoalveolar
lavage fluid (BALF) cytology in 8 horses during an RAO attack induced by
stabling on straw. The results were compared to those obtained from 4
susceptible horses kept in a pasture. Significant changes in cytokine profile
and BALF pattern were observed in the challenge group during the study (12
days) while no modifications were detected in the control group. An increase in
IL-1β mRNA and protein preceded the increase of the same parameters as
IL-8, but the related mRNA and respective protein levels did not strictly
follow the same pattern. The rise in IL-1β protein, but not mRNA, continued over the entire
period and was correlated with BALF neutrophilia. On the contrary, the increase
in both IL-8 mRNA and protein were transient, and the peak of the protein (day
8) followed the peak of BALF neutrophilia. The increase of IL-1β, paralleled by
neutrophil and epithelial cell accumulation in the BALF, is indicative of the
active production of the cytokine by these cells. This reinforces the pivotal
role of cells other than the macrophages in driving airway inflammation during
RAO.
Key Words- Cytokines IL-1β and IL-8 mRNA, proteins,
bronchoalveolar fluids, equine airway obstruction.
FULL-TEXT(SUBSCRIPTION OR PURCHASE TITLE $25USD)