Volume 23 (1):94-101,
2019.
Effect of Enrofloxacin on E. coli, hatchability, omphalitis and
residue in Ross egg yolk and chick tissue
Nibras N Abdulhamza and Orooba MS Ibrahim.
Department
of Physiology, Biochemistry and Pharmacology, College of Veterinary Medicine,
University of Baghdad, Iraq.
ABSTRACT
Abdulhamza NN, Ibrahim OMS., Effect of enrofloxacin on E.
coli , hatchability, omphalitis and residue in Ross egg and chick
tissue, Onl J Vet Res., 23 (1):94-101. Effect of enrofloxacin on growth of pathogenic E. coli isolated
from yolk sac, hatchability, omphalitis and residue in eggs and chick tissue is
described. Minimum inhibitory concentrations (MIC) were determined by broth
dilution assay for Enrofloxacin 30% and Ciprofloxacin 20% (NCCLS 2007). Enrofloxacin
inhibited E coli dose dependently 2µg/ml
MIC 16mm to 10 µg/ml MIC 24mm. Groups of 15 fertile Ross eggs each were then
dipped in 2, 4, 6, 8 and 10 µg/ml enrofloxacin for 3
h at 37C followed by immersion at 4Co to permeate antibiotic through
egg shell pores by temperature differential. Residue of enrofloxacin
in egg yolks and in excised muscle and liver tissue of born chicks was
determined by agar well diffusion test. Untreated controls had hatchability of
73.3% and omphalitis in 37% eggs. Eggs treated with 8 or 10µg/ml enrofloxacin hatched
100% with no omphalitis. 2-4µg/ml enrofloxacin hatched only ~80% preventing omphalitis in ~75%.
10mg/ml in egg yolk declined to
0.2mg/ml by day 17. We found no enrofloxacin in
muscle or liver of 7 day chicks.
Keyword: Enrofloxacin, hatchability, omphalitis, E
.coli, dipping.