©1996-2018. All Rights Reserved. Online Journal of Veterinary Research . You may not store these pages in any form except
for your own personal use. All other usage or distribution is illegal under
international copyright treaties. Permission to use any of
these pages in any other way besides the before mentioned must be gained in
writing from the publisher. This article is exclusively copyrighted in
its entirety to OJVR. This article may be copied once but may not be, reproduced
or re-transmitted without the express permission of the editors. This journal
satisfies the refereeing requirements (DEST) for the Higher Education Research
Data Collection. Linking:To
link to this page or any pages linking to this page you must link directly to
this page only here rather than put up your own page.
OJVRTM
Online Journal of
Veterinary Research©
Volume 18(12): 941-963,
2014. Redacted
2018.
Suspected selective susceptibility to endotoxin in
an ovine model
Saul Chemonges BVetMed, PGDipVetClinSt, MVSt, MANZCVS.
School of Veterinary Science, The
University of Queensland, Gatton, QLD 4343, Australia. Central Analytical Research Facility,
Institute for Future Environments, Queensland University of Technology, Gardens
Point, George Street, Brisbane QLD 4001, Australia.
ABSTRACT
Chemonges S.,
Suspected selective susceptibility to endotoxin in an ovine model, Onl J Vet Res., 18(12): 941-963, 2014. Recent observations show that some sheep appear to be
more susceptible to the effects of Escherichia coli lipopolysaccharide
(LPS) endotoxin than others despite being the same age and breed. This
incidental observation made during a study with a translational benefit to
human emergency medicine prompted further investigations focused on the
background of the base model (sheep) and peri-experimental
practices. A predetermined dose of 15 µg/kg of LPS from E.
coli serotype O55:B5 was planned to be infused into a number of
sheep to prime their immune system prior to blood transfusion studies. Some
sheep subsequently received a lesser dose of LPS due to unexpected heighted
susceptibility to endotoxin. It was hypothesised
that genetic, environmental
or managemental practices
could have been
contributing factors to
this observation. Sheep that were more robust to endotoxin were raised
in open pasture, whereas the more susceptible ones were essentially from
a controlled scientific breeding colony. Epigenetic factors need to be
considered when designing protracted large animal experiments as these aspects
can influence the host’s response to endotoxin challenge. It is suggested that
an understanding of the proteogenomics of serum or
plasma could help to understand LPS morbidity in sheep and similar pathology in
other mammals, including humans.
KEY-WORDS: Selective resistance to endotoxin; Sheep; Escherichia coli;
Lipopolysaccharide (LPS); disease
challenge; Large animal models; serum and plasma proteogenomics.
FULL-TEXT (SUBSCRIPTION
OR PURCHASE TITLE $25USD)