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OJVRTM
Online Journal of Veterinary Research©
(Including Medical and Laboratory Research)
Established 1994
ISSN 1328-925X
Volume 26 (10): 747-751, 2022.
ELISA to differentiate avian influenza virus from
virus in vaccinated poultry.
Talazadeh F, Mayahi
M, Seifi M.
Faculty of
Veterinary Medicine, Shahid Chamran
University of Ahvaz, Iran
ABSTRACT
Talazadeh F, Mayahi M, Seifi M., ELISA to
differentiate avian influenza virus from virus in vaccinated poultry, Onl J Vet Res., 26
(10): 747-751, 2022. We tested an
ELISA to differentiate avian influenza virus from that
in infected poultry. Viruses
were isolated from 10-day-old embryonated chicken
eggs sub typed by hemagglutination
and neuraminidase-inhibition tests with polyclonal chicken antisera. Viruses
were passaged in eggs for allantoic fluids harvested
as stock virus. Subtype H9N2 was used for inactivated vaccine. Then, 2 groups of 100 chicks each were vaccinated
subcutaneously in neck at day 21 with vaccine or infected intra-nasally with 0.2ml allantoic fluid. One hundred controls were not vaccinated
or inoculated virus. Blood for sera was taken at day 42 for commercial ELISA
kit assay in u-bottomed 96-well microtiter plates. Hemagglutination inhibition
was done in U-bottomed 96-well microtiter plates
with 5% chicken erythrocytes. By
hemagglutinin test reference sensitivity and specificity of ELISA was 100%. Three
weeks after infection or vaccination, sera tested positive but we found no
significant differences in viruses between infected and vaccinated poultry.
Key words: avian
influenza virus (AIV), DIVA,
commercial ELISA.
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