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Online Journal of Veterinary Research

Established 1994

ISSN 1328-925X


Volume 22 (10):888-900, 2018.

Histopathology in rabbits injected sonicated Klebsiella and Eimeria antigens challenged with virulent Klebsiella pneumonia.

Basil Razook Faraj Razook.


Zoonoses Unit, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq.




Faraj Razook BR., Histopathology in rabbits injected sonicated Klebsiella and Eimeria antigens challenged with virulent Klebsiella pneumonia, Onl J Vet Res., 22 (10):888-900, 2018. Author describes microscopic findings in rabbits injected Klebsiella pneumonia with or without Eimeria tenella antigens and then challenged with virulent Klebsiella pneumonia. Four groups of 4 rabbits each were injected 1ml of 1000 g/ml sonicated Klebsiella pneumonia antigen, Eimeria tenella, or 500 g/ml or Klebsiella pneumonia with 500 g/ml Eimeria tenella antigens. Controls were injected subcutaneously 1ml phosphate buffer saline (PBS) instead of antigen. At 14 days, rabbits were then given booster shots and all (including controls) rabbits challenged at 60 days with an intraperitoneal injection of 7.5x105CFU/ml virulent Klebsiella pneumonia. Rabbits were sacrificed 7 days after for liver, kidney and lung tissue microscopy. Pathology induced by Klebsiella and Eimeria antigens alone or combined appeared different: In lungs of rabbits injected Klebsiella, there was inflammatory white cell infiltration (WCI) in interstitial tissues, severe collapse of alveoli, hypertrophy of arterial walls and vascular congestion (VC), in liver, VC and dilated sinusoids with WCI, in spleen WCI, but in kidneys, only dilated tubules. In contrast, in those injected Eimeria, lungs sections appeared collapsed with exudate in bronchioles, liver hepatocytes had fatty change displacing nuclei and apoptosis. The spleen showed moderate WCI, but in kidneys we found hemorrhage with VC. In rabbits injected BOTH antigens, lungs were infiltrated with WCI but with fibrin between alveoli and degeneration/necrosis of epithelial cells of the bronchi. In liver, hepatocytes were surrounded by WCI with VC. The spleen showed similar lesions to those described. In kidneys we observed dilation of renal tubules and WCI in interstitial tissue. In PBS controls but challenged with virulent Klebsiella we found mild emphysema with WCI in alveoli and VC. In the liver we also found apoptosis in hepatocytes and similar fatty change as described above. However in the spleen we observed depletion of lymphoid tissues with arterial stenosis of artery lumen and in the kidney we found dilation of bowman's capsule and degeneration of epithelial cells of tubules, and neutrophils in interstitial tissue.


Keywords: Klebsiella, pneumonia, Eimeria, tenella, Rabbits, Immunization.