BACK TO MAIN PAGE


©1994-2018. All Rights Reserved. Online Journal of Veterinary Research. You may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety to OJVR publications. This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors.


OJVRTM

 

Online Journal of Veterinary Research©

 

Volume 9 (1) : 12-18, 2005. Redacted 2018.


 Isolation of egg drop syndrome virus and protein fractionation analysis using sodium dodecyl sulphate polyacrylamide gel electrophoresis

  

Rasool MH, Rahman SU, Mansoor MK, Siddique M

 

Molecular Virology Laboratory Department of Veterinary Microbiology, University of Agriculture, Faisalabad- 38040, Pakistan

 

ABSTRACT

 

Rasool MH, Rahman SU, Mansoor MK, Siddique M., Isolation of Egg Drop Syndrome Virus and Protein Fractionation Analysis using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis Onl J Vet Res., 9 (1) : 12-18, 2005. Six isolates of egg drop syndrome (EDS) virus were recovered from 5 outbreaks of EDS in commercial laying hens. Aberrant eggs were fed to susceptible laying hens to induce infection and tissue samples were inoculated in 11 days old duck embryos. Virus in harvested allanto-amniotic fluid was verified by spot and micro hemaglutination inhibition and agar gel precipitation tests. The EDS virus grew in duck embryo and agglutinated avian but not mammalian red blood cells. Isolates were purified through velocity density gradient centrifugation. Protein concentration was determined by the Lowry method and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) was conducted by loading 300µg protein concentration on 12.5% gel using discontinuous buffer system. The six isolates showed 13 polypeptides, which were identical to those described in the referral EDS-76 virus (strain-127). The molecular weights of the polypeptides ranged from 6.5 KDa to 126 KDa.

 

KEY WORDS: Egg Drop Syndrome, Haemagglutination, Oviduct, Duck embryos, Sodium dodecyl sulphate polyacrylamide gel electrophoresis, polypeptides.


 

BACK TO MAIN PAGE

 

FULL-TEXT (SUBSCRIPTION OR PURCHASE ARTICLE)