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OJVRTM

Online Journal of Veterinary Research

Volume 24 (6):356-365, 2020.


Histopathology and F1L gene sequence analysis of contagious ecthyma virus in sheep and goats.

 

Davari SA1 (DVM, PhD), Sayyari M2* (DVM, PhD), Mohammadi A2 (DVM, PhD)

 

1Department(s) of Pathobiology, Faculties of Veterinary Medicine, Zabol University, Zabol, 2Shiraz University, Shiraz, Iran

 

ABSTRACT

 

Davari SA, Sayyari M, Mohammadi A., Histopathology and F1L gene sequence analysis of contagious ecthyma virus in sheep and goats, Onl J Vet Res., 24 (6):356-365, 2020. Contagious ecthyma, also known as Orf, is a common viral skin disease of sheep and goats caused by Parapoxvirus. We describe pathology and Orf virus F1L gene sequence by PCR from sheep and goats. Tissue samples revealed epidermal hyperplasia with prominent rete ridges, hydropic degeneration of the necrotic keratinocytes, eosinophilic intracytoplasmic inclusion bodies in vacuolated cells and subcorneal pustules. By PCR we found ~50% positive to the virus of which 10 were sequenced for Orf-059-Shiraz strain recorded in GeneBank for phylogenetic analysis and suggested that this gene is highly conserved.

 

Key words: Contagious ecthyma, Histopathology, Molecular analysis.


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