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OJVRTM
Online Journal of Veterinary
Research ©
Volume 15 (5): 446-454, 2011. Redacted 2017.
Molecular epidemiology of virotypes of Escherichia coli in feces
of healthy household cats.
Ghanbarpour Reza1 (DVM,
PhD), Akhtardanesh Baharak2 (DVM, PhD), Pakseresht Akram2 (DVM).
1Microbiology, 2Small Animal Internal Medicine Departments of
Faculty of Veterinary Medicine, Shahid Bahonar University, 76169-14111, Kerman, Iran.
ABSTRACT
Reza G, Baharak A, Akram P., Molecular epidemiology of virotypes
of Escherichia coli in feces of healthy household
cats, Onl J Vet Res., 15 (5): 446-454, 2011. Escherichia
coli strains are classified on
the basis of their virulence genotypes into virotypes. Sixty nine E.
coli isolates from feces of healthy household
cats were examined to determine a selection of virulence genes.
Phylogenetic groups and subgroups of the isolates were determined by PCR. In 30 (43.47%) E. coli isolates virulence genes
were present alone or in combination with each other. The
detected virulence genes were classified in sixteen different patterns, which
were considered as different virotypes. The most common virulence gene was sfa/focD-E, which
was positive in 18 (26.08%) isolates. Among the E. coli isolates, 24.63% had hly, 14.49%
iucD, 14.49% papE-F, 13.04% cnf1, 1.44% stx2 and 1.44% eae
genes. Ten combination patterns of the virulence genes were detected in
examined isolates, which combination of hly, sfa, pap, cnf1 genes was the
most prevalent pattern. None of the
isolates contained the afaIB-C, stx1,
STI, LTI, cnf2 and ipaH genes. Virotyps were distributed in four main phylogroups.
Sixty nine isolates belonged to A (60.87%), B1 (17.39%), B2 (7.24%) and D
(14.50%) phylogroups. Twelve isolates fell in to B1 group,
whereas 57 isolates (82.61%) fell into five phylogenetic subgroups: 23.19% into
A0, 37.68% into A1, 7.24% into B22, 10.14%
into D1 and 4.35% into D2. None of the isolates belonged
to B23 subgroup. In conclusion, virulence genes were found in E. coli isolates from feces of healthy household cats. Virotypes were differed phylogenetically and could be excreted into the external environment and transmitted to
susceptible hosts.
Key words: Escherichia
coli; virotype,
genotype, cat.
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