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Online Journal of Veterinary Research

(Including Medical and Laboratory Research)

Established 1994
ISSN 1328-925X


Volume 27 (8): 467-471, 2023.

Antimicrobial and antifungal effects of Echinacea purpurea and Thymus vulgaris extracts on food pathogens.


Hamid Reza Gheisari1, Hassan Habibi2.


1Food Hygiene Department and 2Avian Diseases Research Center, School of Veterinary Medicine, Shiraz University, Iran




Gheisari HR, Habibi H., Antimicrobial effects of Echinacea purpurea and Thymus vulgaris extracts on food pathogens, Onl J Vet Res., 27 (8): 467-471, 2023. Antibacterial activity of methanolic extracts of Echinacea purpurea and Thymus vulgaris on food pathogens were investigated. Air-dried plant materials were ground to fine powder and extracted by Soxhelet apparatus with methanol. The extract was tested at a concentration of 20mg/ml against a panel of food pathogen bacteria and fungi by tube dilution. For T. vulgaris Minimum inhibitory concentrations (MIC) varied 0.625-2.5 and E. purpura 0.31--2.5mg/ml. Extracts had same MIC against Listeria monocytogenes and Staphylococcus aureus whereas T. vulgaris extract were more effective against Salmonella typhimorium and Escherichia coli O157: H7. Bacillus subtilis and Proteus vulgaris were inhibited by E. purpurea. Only 16 times dose of T vulgaris inhibited fungi Aspergilosis flavus (MIC = 40 mg/ml, fungal inhibition = 160 mg/ml). We find that extracts exhibited antibacterial effects but had no antifungal activity.


Key words: Echinacea purpurea; Thymus vulgaris; Antimicrobial activities; Food pathogens.