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Online Journal of Veterinary Research

Volume 24 (5):290-293, 2020.

Avian influenza H9N2 virus in lung and kidney of broilers 3, 7, 11 and 15 days post inoculation.


Tavakkoli Hadi


Department of Avian Medicine, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran




Hadi T., Replication of avian influenza H9N2 virus in lung and kidney of broilers 3, 7, 11 and 15 days post inoculation, Onl J Vet Res., 24 (5):290-293, 2020. Avian influenza is a contagious viral disease of wild and domestic birds. Groups of 25 day-old commercial (AA plus) 30 day old AA plus broilers were challenged intranasally allantoic fluid with 106EID50/100l H9N2 virus. Birds were tested for H9N2 antibodies in blood by hemagglutination inhibition test and lung and kidney tissue by HT-PCR at 3, 7, 11 and 15 days after challenge. Another 25 birds were used as unchallenged controls. Clinical signs were monitored daily for the 15 days and at necropsy 5 chickens from challenge and controls groups were randomly selected for pathology. In 2/5 (40%) lungs or 4/5 (80%) kidneys of infected chicks we found most viral RNA on day 7. Other days varied 0/5 to 1/5 but mostly in kidneys (Table 2). Lungs had cleared by day 15. Clinical signs in challenged chicks were conjunctivitis, depression, excessive lacrimation, ruffled feathers, decreased feed consumption, coughing and sneezing. Signs were apparent by day 3 but most obvious at Day 7 correlating with viral loads. By day 11 clinical signs reduced and disappeared by day 15. At necropsy we observed hemorrhage in small intestine, congestion of trachea, air sacculitis and swollen kidneys.


Keywords: Avian influenza, Broiler, H9N2, Low pathogenic avian influenza, RT-PCR.